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PCR past, present and future
- Source :
- Biotechniques
- Publication Year :
- 2020
-
Abstract
- PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.
- Subjects :
- 2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19)
Computer science
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Point-of-Care Systems
Pneumonia, Viral
microfluidics
Computational biology
Review
01 natural sciences
Polymerase Chain Reaction
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Betacoronavirus
COVID-19 Testing
reverse transcription PCR
Animals
Humans
Digital polymerase chain reaction
Pandemics
Biomedicine
030304 developmental biology
0303 health sciences
business.industry
Clinical Laboratory Techniques
SARS-CoV-2
digital PCR
RNA virus diagnoses
010401 analytical chemistry
COVID-19
Equipment Design
Microfluidic Analytical Techniques
0104 chemical sciences
Reverse transcription polymerase chain reaction
PCR
portable systems
Pcr method
business
Coronavirus Infections
point-of-care diagnostics
Biotechnology
Subjects
Details
- ISSN :
- 19409818
- Volume :
- 69
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- BioTechniques
- Accession number :
- edsair.doi.dedup.....ade682d1f49a94d5888b648d3b617f35