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TACC3 mediates the association of MBD2 with histone acetyltransferases and relieves transcriptional repression of methylated promoters
- Source :
- Europe PubMed Central, Nucleic Acids Research
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Abstract
- We have recently reported that a novel MBD2 interactor (MBDin) has the capacity to reactivate transcription from MBD2-repressed methylated promoters even in the absence of demethylation events. Here we show that another unrelated protein, TACC3, displays a similar activity on methylated genes. In addition the data reported here provide possible molecular mechanisms for the observed phenomenon. Immunoprecipitation experiments showed that MBD2/TACC3 form a complex in vivo with the histone acetyltransferase pCAF. MBD2 could also associate with HDAC2, a component of MeCP1 repression complex. However, we found that the complexes formed by MBD2 with TACC3/pCAF and with HDAC2 were mutually exclusive. Moreover, HAT enzymatic assays demonstrated that HAT activity associates with MBD2 in vivo and that such association significantly increased when TACC3 was over-expressed. Overall our findings suggest that TACC3 can be recruited by MBD2 on methylated promoters and is able to reactivate transcription possibly by favoring the formation of an HAT-containing MBD2 complex and, thus, switching the repression potential of MBD2 in activation even prior to eventual demethylation.
- Subjects :
- Transcriptional Activation
Transcription, Genetic
Biology
DNA-binding protein
Article
Transcription (biology)
Two-Hybrid System Techniques
transcriptional repression
Genetics
Animals
Humans
Immunoprecipitation
Gene Silencing
Promoter Regions, Genetic
Psychological repression
Histone Acetyltransferases
Sequence Deletion
Cell Nucleus
Centrosome
Histone deacetylase 2
Promoter
DNA Methylation
Molecular biology
Corrigenda
DNA-Binding Proteins
TACC3
PCAF
DNA methylation
Microtubule-Associated Proteins
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Europe PubMed Central, Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....adedb32254b03b13806bc5e7bddc02fa