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Regulation of glial size by eicosapentaenoic acid through a novel Golgi apparatus mechanism
- Source :
- PLoS Biology, Vol 18, Iss 12, p e3001051 (2020), PLoS Biology
- Publication Year :
- 2020
- Publisher :
- Public Library of Science (PLoS), 2020.
-
Abstract
- Coordination of cell growth is essential for the development of the brain, but the molecular mechanisms underlying the regulation of glial and neuronal size are poorly understood. To investigate the mechanisms involved in glial size regulation, we used Caenorhabditis elegans amphid sheath (AMsh) glia as a model and show that a conserved cis-Golgi membrane protein eas-1/GOLT1B negatively regulates glial growth. We found that eas-1 inhibits a conserved E3 ubiquitin ligase rnf-145/RNF145, which, in turn, promotes nuclear activation of sbp-1/ SREBP, a key regulator of sterol and fatty acid synthesis, to restrict cell growth. At early developmental stages, rnf-145 in the cis-Golgi network inhibits sbp-1 activation to promote the growth of glia, and when animals reach the adult stage, this inhibition is released through an eas-1-dependent shuttling of rnf-145 from the cis-Golgi to the trans-Golgi network to stop glial growth. Furthermore, we identified long-chain polyunsaturated fatty acids (LC-PUFAs), especially eicosapentaenoic acid (EPA), as downstream products of the eas-1-rnf-145-sbp-1 pathway that functions to prevent the overgrowth of glia. Together, our findings reveal a novel and potentially conserved mechanism underlying glial size control.<br />The molecular mechanisms underlying the regulation of glial and neuronal size are poorly understood. This study in nematodes reveals eicosapentaenoic acid as the downstream product of a pathway that functions to prevent the overgrowth of glia, suggesting a novel and potentially conserved mechanism underlying glial size control.
- Subjects :
- Embryology
Nematoda
Golgi Apparatus
Biochemistry
Infographics
chemistry.chemical_compound
Mathematical and Statistical Techniques
RNA interference
Animal Cells
Biology (General)
Caenorhabditis elegans
Data Management
Neurons
biology
General Neuroscience
Statistics
Fatty Acids
Eukaryota
Animal Models
Lipids
Ubiquitin ligase
Cell biology
Nucleic acids
Phenotypes
Genetic interference
Experimental Organism Systems
Eicosapentaenoic Acid
Caenorhabditis Elegans
Physical Sciences
symbols
Fatty Acids, Unsaturated
Epigenetics
Cellular Types
General Agricultural and Biological Sciences
Graphs
Neuroglia
Research Article
Computer and Information Sciences
QH301-705.5
Ubiquitin-Protein Ligases
Research and Analysis Methods
General Biochemistry, Genetics and Molecular Biology
symbols.namesake
Model Organisms
Genetics
Animals
Statistical Methods
Caenorhabditis elegans Proteins
Fatty acid synthesis
Cell Size
Analysis of Variance
General Immunology and Microbiology
Biology and life sciences
Cell growth
Data Visualization
Embryos
Organisms
Cell Biology
Golgi apparatus
biology.organism_classification
Invertebrates
Sterol regulatory element-binding protein
Membrane protein
chemistry
Cellular Neuroscience
biology.protein
Animal Studies
Caenorhabditis
RNA
Gene expression
Zoology
Mathematics
Neuroscience
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 15457885 and 15449173
- Volume :
- 18
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS Biology
- Accession number :
- edsair.doi.dedup.....ae062bac33fcfc8fbefe606fdcb89eb8