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Acetone-butanol-ethanol (ABE) fermentation of soluble and hydrolyzed sugars in apple pomace by Clostridium beijerinckii P260
- Source :
- Fuel. 244:536-544
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The decreasing supply of fossil fuels and increasing environmental concern of food waste disposal have raised interests in food waste conversation to biofuels such as butanol. Apple pomace, a food processing waste rich in carbohydrates, is a good feedstock for butanol production. The goal of this study is to present and evaluate a process to thoroughly convert apple pomace water soluble sugars (WSS) and hydrolyzed sugars from structural carbohydrates to acetone-butanol-ethanol (ABE) by fermentation. WSS was extracted from apple pomace by hot water. The solid residue was pretreated with acid or alkali followed by enzymatic hydrolysis to obtain acid hydrolyzed sugars (ACHS) or alkali hydrolyzed sugars (ALHS). Finally, WSS, ACHS, ALHS, WSS + ACHS, and WSS + ALHS were used as substrates to produce ABE by Clostridium beijerinckii P260, respectively. Acid and alkali pretreated apple pomace showed significantly (p < 0.05) higher glucose yield after cellulase hydrolysis compared with that of unpretreated apple pomace. Addition of pectinase increased hydrolyzed glucose yield by 27.9%, 26.9%, and 33.0% for acid pretreated sample, alkali pretreated sample, and unpretreated sample, respectively. Fermentation results revealed that inhibitors generated during pretreatment could negatively affect the ABE fermentation rate and titers; however, this negative effect could be alleviated by mixing the hydrolyzed sugars with water soluble sugars. A total of 202.8, 42.1, 41.4, 260.1, and 262.2 g of ABE was produced from each kg of dry apple pomace using WSS, ACHS, ALHS, WSS + ACHS, and WSS + ALHS as the substrates, respectively, based on the mass balance. USDA AFRI Foundational Program [2018-67021-27895]; Virginia Agriculture Experiment Station; Hatch Program of the National Institute of Food and Agriculture (NIFA), USDA This work was supported by the USDA AFRI Foundational Program (grant number 2018-67021-27895) and the Virginia Agriculture Experiment Station and the Hatch Program of the National Institute of Food and Agriculture (NIFA), USDA. Public domain – authored by a U.S. government employee
- Subjects :
- Sodium hydroxide pretreatment
020209 energy
General Chemical Engineering
Energy Engineering and Power Technology
02 engineering and technology
complex mixtures
chemistry.chemical_compound
Hydrolysis
020401 chemical engineering
Enzymatic hydrolysis
0202 electrical engineering, electronic engineering, information engineering
Acetone-butanol-ethanol (ABE)
Food science
0204 chemical engineering
biology
Food waste
Butanol
Organic Chemistry
Pomace
Sulfuric acid pretreatment
equipment and supplies
biology.organism_classification
Fuel Technology
Clostridium beijerinckii
chemistry
Biofuel
Fermentation
cardiovascular system
bacteria
circulatory and respiratory physiology
Subjects
Details
- ISSN :
- 00162361
- Volume :
- 244
- Database :
- OpenAIRE
- Journal :
- Fuel
- Accession number :
- edsair.doi.dedup.....ae1781edc8e032c3f78e48cf752c7305