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A Brassica oleracea gene expressed in a variety-specific manner may encode a novel plant transmembrane receptor
- Source :
- RIUR: Repositorio Institucional de la Universidad de La Rioja, Universidad de La Rioja (UR), RIUR. Repositorio Institucional de la Universidad de La Rioja, instname, Digital.CSIC. Repositorio Institucional del CSIC
- Publication Year :
- 2001
-
Abstract
- The species Brassica oleracea includes several agricultural varieties characterized by the proliferation of different types of meristems. Using a combination of subtractive hybridization and PCR (polymerase chain reaction) techniques we have identified several genes which are expressed in the reproductive meristems of the cauliflower curd (B. oleracea var. botrytis) but not in the vegetative meristems of Brussels sprouts (B. oleracea var. gemmifera) axillary buds. One of the cloned genes, termed CCE1 (CAULIFLOWER CURD EXPRESSION 1) shows specific expression in the botrytis variety. Preferential expression takes place in this variety in the meristems of the curd and in the stem throughout the vegetative and reproductive stages of plant growth. CCE1 transcripts are not detected in any of the organs of other B. oleracea varieties analyzed. Based on the nucleotide sequence of a cDNA encompassing the complete coding region, we predict that this gene encodes a transmembrane protein, with three transmembrane domains. The deduced amino acid sequence includes motifs conserved in G-protein-coupled receptors (GPCRs) from yeast and animal species. Our results suggest that the cloned gene encodes a protein belonging to a new, so far unidentified, family of transmembrane receptors in plants. The expression pattern of the gene suggests that the receptor may be involved in the control of meristem development/arrest that takes place in cauliflower.<br />This research was supported by Salem-Teikyo University Graduate Research funds and by a Research Initiation Grant to M.C.-A. from the NASA-West Virginia Space Grant Consortium.
- Subjects :
- DNA, Complementary
G-protein
Transcription, Genetic
Physiology
Molecular Sequence Data
Restriction Mapping
G-protein-coupled receptors
Receptors, Cell Surface
Plant Science
Brassica
Biology
Polymerase Chain Reaction
Protein Structure, Secondary
Meristems
Cauliflowers
Species Specificity
Gene Expression Regulation, Plant
Coupled receptors
Complementary DNA
Amino Acid Sequence
Cloning, Molecular
Peptide sequence
Gene
DNA Primers
Plant Proteins
Regulation of gene expression
Genetics
Endodeoxyribonucleases
Base Sequence
Plant Stems
Sequence Homology, Amino Acid
Holliday Junction Resolvases
food and beverages
Cell Biology
General Medicine
Cauliflower
biology.organism_classification
Transmembrane proteins
Transmembrane protein
Recombinant Proteins
Transmembrane domain
Suppression subtractive hybridization
Brassica oleracea
Sequence Alignment
Subjects
Details
- ISSN :
- 00320781
- Volume :
- 42
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Plantcell physiology
- Accession number :
- edsair.doi.dedup.....ae4cb4597e7217c287c77880d7429309