Back to Search
Start Over
VHH against DARC
- Source :
- Cellular and Molecular Life Sciences, Cellular and Molecular Life Sciences, Springer Verlag, 2010, 67 (19), pp.3371-87. ⟨10.1007/s00018-010-0387-6⟩
- Publication Year :
- 2010
- Publisher :
- Birkhauser Verlag Basel, 2010.
-
Abstract
- International audience; Fy blood group antigens are carried by the Duffy antigen receptor for chemokines (DARC), a red cells receptor for Plasmodium vivax broadly implicated in human health and diseases. Recombinant VHHs, or nanobodies, the smallest intact antigen binding fragment derivative from the heavy chain-only antibodies present in camelids, were prepared from a dromedary immunized against DARC N-terminal extracellular domain and selected for DARC binding. A described VHH, CA52, does recognize native DARC on cells. It inhibits P. vivax invasion of erythrocytes and displaces interleukin-8 bound to DARC. The targeted epitope overlaps the well-defined DARC Fy6 epitope. K (D) of CA52-DARC equilibrium is sub-nanomolar, hence ideal to develop diagnostic or therapeutic compounds. Immunocapture by immobilized CA52 yielded highly purified DARC from engineered K562 cells. This first report on a VHH with specificity for a red blood cell protein exemplifies VHHs' potentialities to target, to purify, and to modulate the function of cellular markers.
- Subjects :
- Erythrocytes
MESH: Immunoglobulins
Plasmodium vivax
Epitope
law.invention
MESH: Recombinant Proteins
0302 clinical medicine
law
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Duffy antigen receptor for chemokines
MESH: Animals
Receptor
MESH: Receptors, Cell Surface
Cancer
0303 health sciences
biology
MESH: Erythrocytes
MESH: Camels
MESH: Receptors, Antigen
MESH: Chemokines
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
Recombinant Proteins
3. Good health
MESH: Plasmodium vivax
medicine.anatomical_structure
030220 oncology & carcinogenesis
Blood Group Antigens
Recombinant DNA
Molecular Medicine
[SDV.IMM]Life Sciences [q-bio]/Immunology
Receptors, Chemokine
Chemokines
Antibody
Camelus
Immunoglobulins
Receptors, Cell Surface
MESH: Carrier Proteins
VHH
Article
03 medical and health sciences
Cellular and Molecular Neuroscience
HIV
Antigen
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
medicine
Animals
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
atypical chemokine receptor
Molecular Biology
030304 developmental biology
Pharmacology
MESH: Humans
MESH: Blood Group Antigens
Interleukin-8
Cell Biology
biology.organism_classification
Virology
Molecular biology
MESH: Interleukin-8
MESH: Duffy Blood-Group System
immunoaffinity
Receptors, Antigen
Red blood cell
inflammation
biology.protein
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
Carrier Proteins
Duffy Blood-Group System
MESH: Receptors, Chemokine
K562 cells
Subjects
Details
- Language :
- English
- ISSN :
- 1420682X and 14209071
- Database :
- OpenAIRE
- Journal :
- Cellular and Molecular Life Sciences, Cellular and Molecular Life Sciences, Springer Verlag, 2010, 67 (19), pp.3371-87. ⟨10.1007/s00018-010-0387-6⟩
- Accession number :
- edsair.doi.dedup.....ae7ca51cba58e19ac3b07c8470d78569