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TIS111D can affect bladder cancer cells by regulating epithelial-mesenchymal transition
- Source :
- Life Sciences. 235:116832
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Aims Delineates the role of TIS111D in bladder cancer. Materials and methods The expression of TIS111D in bladder cancer and adjacent tissues was assessed by immunohistochemistry, Western blot and real-time PCR. Western blot and real-time PCR were used to analyse the expression of TIS111D in HT1197, T24, 5637 and TCCSUP cells. After TIS111D was silenced in T24, 5637 and TCCSUP cells, MTT and Transwell assays were used to detect the effects of TIS111D on proliferation and migration. Western blot and real-time PCR were used to detect the regulatory effect of downregulation of TIS111D on N-cad and E-cad. In vivo experiments confirmed the role of TIS111D in the growth and migration of bladder cancer and determined whether the role of TIS111D in bladder cancer is related to its regulation of N-cad and E-cad. Key findings The expression of TIS11D was higher in tumour tissues and bladder cancer cells. Si-TIS111D could inhibit the growth and migration of bladder cancer cells, while TIS111D could regulate the expression of E-cad and N-cad to regulate epithelial-mesenchymal transition (EMT). We also demonstrated that TIS111D could promote the growth and migration of bladder cancer in vivo by regulating EMT. Significance TIS111D may participate in the regulation of bladder cancer progression by regulating EMT.
- Subjects :
- 0301 basic medicine
Epithelial-Mesenchymal Transition
Down-Regulation
Biology
urologic and male genital diseases
030226 pharmacology & pharmacy
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Western blot
Cell Movement
Transcription Factor TFIIIB
In vivo
Cell Line, Tumor
medicine
Humans
Epithelial–mesenchymal transition
RNA, Small Interfering
General Pharmacology, Toxicology and Pharmaceutics
Cell Proliferation
Bladder cancer
Transition (genetics)
medicine.diagnostic_test
General Medicine
Cadherins
medicine.disease
030104 developmental biology
Urinary Bladder Neoplasms
Cancer research
Immunohistochemistry
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 235
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....ae8d290e1bbb85bc7c53b3b889c4e65d