Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging

Authors :: Ranieri Bizzarri
Enrico Gratton
Barbara Storti
Michelle A. Digman
Fabio Beltram
Antonella Battisti
Battisti, A
Digman, Ma
Gratton, E
Storti, B
Beltram, Fabio
Bizzarri, R.
Source :: Battisti, A; Digman, MA; Gratton, E; Storti, B; Beltram, F; & Bizzarri, R. (2012). Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging. Chemical Communications, 48(42), 5127-5129. doi: 10.1039/c2cc30373f. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/2j42t0qd, Chemical communications (Lond., 1996, Print) 48 (2012): 5127–5129. doi:10.1039/c2cc30373f, info:cnr-pdr/source/autori:Battisti, A; Digman, MA; Gratton, E; Storti, B; Beltram, F; Bizzarri, R/titolo:Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging/doi:10.1039%2Fc2cc30373f/rivista:Chemical communications (Lond., 1996, Print)/anno:2012/pagina_da:5127/pagina_a:5129/intervallo_pagine:5127–5129/volume:48
Publication Year :: 2012
Abstract: A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy. © 2012 The Royal Society of Chemistry.

Language :: English
Database :: OpenAIRE
Journal :: Battisti, A; Digman, MA; Gratton, E; Storti, B; Beltram, F; & Bizzarri, R. (2012). Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging. Chemical Communications, 48(42), 5127-5129. doi: 10.1039/c2cc30373f. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/2j42t0qd, Chemical communications (Lond., 1996, Print) 48 (2012): 5127–5129. doi:10.1039/c2cc30373f, info:cnr-pdr/source/autori:Battisti, A; Digman, MA; Gratton, E; Storti, B; Beltram, F; Bizzarri, R/titolo:Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging/doi:10.1039%2Fc2cc30373f/rivista:Chemical communications (Lond., 1996, Print)/anno:2012/pagina_da:5127/pagina_a:5129/intervallo_pagine:5127–5129/volume:48
Accession number :: edsair.doi.dedup.....ae9b1a66344dfcc167c7fda434a75be1
Full Text :: https://doi.org/10.1039/c2cc30373f.

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