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MWCNTs-CTAB and HFs-Lac Nanocomposite-Modified Glassy Carbon Electrode for Rutin Determination
- Source :
- Biosensors; Volume 12; Issue 8; Pages: 632
- Publication Year :
- 2022
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2022.
-
Abstract
- Rutin is a flavonoid glycoside compound, which is mainly transported via the blood circulation system in the human body. The monitoring of the blood concentration of rutin is of great significance in many fields such as pharmacology and pharmacokinetics. In this work, a biosensor based on multi-walled carbon nanotubes (MWCNTs), cetyltrimethylammonium bromide (CTAB), hydroxyl fullerenes (HFs), and laccase (Lac) nanocomposite-modified glassy carbon electrodes was constructed. The modified materials were characterized with a transmission electron microscope (TEM), cyclic voltammograms (CV), and electrochemical impedance spectroscopy (EIS). CTAB is used to disperse MWCNTs and improve hydrophilicity and biocompatibility of MWCNTs, while the use of Lac can enhance the oxidation of catechol structure in rutin, thus significantly improving the sensitivity and selectivity of the modified electrode. Linear sweep voltammetry (LSV) studies showed that the determination linear ranges of rutin were 0.1 µmol L−1 to 2 µmol L−1 and 2 µmol L−1 to 11 µmol L−1, with the determination limits of 30 nmol L−1 and 95.5 nmol L−1, respectively. The proposed biosensor can be used to detect rutin tablets and serum samples with high recovery, which indicates a good accuracy of this method, and the results are consistent with those measured by the traditional ultra-high performance liquid chromatography (UHPLC) method. Hence, this biosensor has potential practical application value in rutin drug quality testing and clinical blood drug concentration monitoring.
- Subjects :
- Cetrimonium
Nanotubes, Carbon
Rutin
Laccase
Clinical Biochemistry
Biomedical Engineering
Electrochemical Techniques
General Medicine
biosensor
laccase
hydroxyl fullerenes
multi-walled carbon nanotubes
rutin determination
Nanocomposites
Analytical Chemistry
Humans
Fullerenes
Electrodes
Instrumentation
Engineering (miscellaneous)
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 20796374
- Database :
- OpenAIRE
- Journal :
- Biosensors; Volume 12; Issue 8; Pages: 632
- Accession number :
- edsair.doi.dedup.....af123d331415fad62b5e02d523324a95
- Full Text :
- https://doi.org/10.3390/bios12080632