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Thymic DCs derived IL-27 regulates the final maturation of CD4+ SP thymocytes
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- IL-27, as a pleiotropic cytokine, promotes the differentiation of naïve T cells to Th1, while suppressing Th2 and Th17 differentiation in the periphery. However, the role of IL-27 in the thymocyte development remains unknown. Here we showed that IL-27 was highly expressed in thymic plasmacytoid dendritic cells (pDCs) while its receptor expression was mainly detected in CD4+ single-positive (SP) thymocytes. Deletion of the p28 subunit in DCs resulted in a reduction of the most mature Qa-2+ subsets of CD4+ SP T cells. This defect was rescued by intrathymic administration of exogenous IL-27. In vitro differentiation assay further demonstrated that IL-27 alone was able to drive the maturation of the newly generated 6C10+CD69+CD4+ SP cells into Qa-2+ cells. Collectively, this study has revealed an important role of thymic DCs-derived IL-27 in the regulation of the phenotypic maturation of CD4+ SP thymocytes.
- Subjects :
- 0301 basic medicine
CD4-Positive T-Lymphocytes
Interleukin-27
medicine.medical_treatment
Cellular differentiation
Receptor expression
Interferon Regulatory Factor-7
Thymus Gland
Article
Injections
03 medical and health sciences
0302 clinical medicine
Antigen
Antigens, CD
medicine
Animals
Receptor
CD4-positive T cells
Adaptor Proteins, Signal Transducing
Multidisciplinary
Thymocytes
Integrases
Chemistry
CD69
Interleukins
Intracellular Signaling Peptides and Proteins
Proteins
RNA-Binding Proteins
Cell Differentiation
hemic and immune systems
Dendritic Cells
Receptors, Interleukin
In vitro
Cell biology
Mice, Inbred C57BL
Thymocyte
030104 developmental biology
Cytokine
Erratum
Carrier Proteins
Gene Deletion
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....af126f1b48b2f912815d9a03b8ea8b25