Back to Search
Start Over
A Specific Nutrient Combination Attenuates the Reduced Expression of PSD-95 in the Proximal Dendrites of Hippocampal Cell Body Layers in a Mouse Model of Phenylketonuria
- Source :
- Nutrients, Nutrients, Vol 8, Iss 4, p 185 (2016), Nutrients, 8(4):185. MDPI AG
- Publication Year :
- 2015
-
Abstract
- The inherited metabolic disease phenylketonuria (PKU) is characterized by increased concentrations of phenylalanine in the blood and brain, and as a consequence neurotransmitter metabolism, white matter, and synapse functioning are affected. A specific nutrient combination (SNC) has been shown to improve synapse formation, morphology and function. This could become an interesting new nutritional approach for PKU. To assess whether treatment with SNC can affect synapses, we treated PKU mice with SNC or an isocaloric control diet and wild-type (WT) mice with an isocaloric control for 12 weeks, starting at postnatal day 31. Immunostaining for post-synaptic density protein 95 (PSD-95), a post-synaptic density marker, was carried out in the hippocampus, striatum and prefrontal cortex. Compared to WT mice on normal chow without SNC, PKU mice on the isocaloric control showed a significant reduction in PSD-95 expression in the hippocampus, specifically in the granular cell layer of the dentate gyrus, with a similar trend seen in the cornus ammonis 1 (CA1) and cornus ammonis 3 (CA3) pyramidal cell layer. No differences were found in the striatum or prefrontal cortex. PKU mice on a diet supplemented with SNC showed improved expression of PSD-95 in the hippocampus. This study gives the first indication that SNC supplementation has a positive effect on hippocampal synaptic deficits in PKU mice.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
congenital, hereditary, and neonatal diseases and abnormalities
Genotype
Phenylketonurias
Phenylalanine
nutrient combination
phenylketonuria
Hippocampus
lcsh:TX341-641
Striatum
Hippocampal formation
Biology
03 medical and health sciences
Mice
Random Allocation
0302 clinical medicine
Internal medicine
medicine
Animals
Neurotransmitter metabolism
Prefrontal cortex
PSD-95
Nutrition and Dietetics
Dentate gyrus
Brief Report
nutritional and metabolic diseases
Membrane Proteins
synaptic proteins
Animal Feed
Diet
030104 developmental biology
medicine.anatomical_structure
Endocrinology
nervous system
Gene Expression Regulation
Female
Pyramidal cell
lcsh:Nutrition. Foods and food supply
Disks Large Homolog 4 Protein
Guanylate Kinases
030217 neurology & neurosurgery
Food Science
Subjects
Details
- ISSN :
- 20726643
- Volume :
- 8
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....af18635d6f4c8c2d8c6b4283650b9a1e