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In vitro anti-hepatitis B and SARS virus activities of a titanium-substituted-heteropolytungstate
- Source :
- Antiviral Research
- Publication Year :
- 2011
- Publisher :
- Elsevier B.V., 2011.
-
Abstract
- Highlights ► A structural determined heteropolytungstate has been synthesized. ► The heteropolytungstate can efficiently inhibit HBV replication in vitro. ► The heteropolytungstate also shows high anti-SARS virus activity in vitro.<br />A structural determined heteropolytungstate, [K4(H2O)8Cl][K4(H2O)4PTi2W10O40]·NH2OH 1, has been synthesized and evaluated for in vitro antiviral activities against hepatitis B (HBV) and SARS virus. The identity and high purity of compound 1 were confirmed by elemental analysis, NMR, IR analysis and single-crystal X-ray diffraction. The compound 1, evaluated in HepG 2.2.15 cells expressing permanently HBV, significantly reduced the levels of HBV antigens and HBV DNA in a dose-dependent and time-dependent manner. EC50 values were determined to be 54 μM for HBeAg, 61 μM for HBsAg and 2.66 μM for supernatant HBV DNA, as compared to 1671, 1570, 169 μM, respectively, for the commercially-available hepatitis B drug adefovir dipivoxil (ADV). Intracellular cccDNA, pgRNA and HBcAg were also found to be decreased by compound 1 in a concentration-dependent manner. Cytotoxicity results showed that compound 1 has low toxicity in HepG 2 cells with CC50 value of 515.20 μM. The results indicate that compound 1 can efficiently inhibit HBV replication in HepG 2.2.15 cells line in vitro. Additionally, compound 1 also shows high anti-SARS activity at an EC50 of 7.08 μM and toxicity with a CC50 of 118.6 μM against MDCK cells.
- Subjects :
- HBsAg
SARS virus
medicine.disease_cause
Virus Replication
Cytopathogenic Effect, Viral
CC0, maximal noncytotoxic concentration
Chlorocebus aethiops
Adefovir
Hepatitis B e Antigens
Antiviral activity
virus diseases
food and beverages
ADV, adefovir dipivoxil
MDCK, Madin–Darby canine kidney
cccDNA
Hep G2 Cells
Hepatitis B
Tungsten Compounds
Hepatitis B Core Antigens
HBcAg
HBeAg
Severe acute respiratory syndrome-related coronavirus
Vero-E6, African green monkey kidney cells
TI, therapeutic index
EC50, 50% effective concentration
DMEM, Dulbecco’s Modified Eagle’s Medium
medicine.drug
Hepatitis B virus
Heteropolytungstate
EC90, 90% effective concentration
Microbial Sensitivity Tests
Biology
Antiviral Agents
Article
FBS, fetal bovine serum
In vitro
Virology
medicine
Animals
Humans
SARS, severe acute respiratory syndrome
CC50, 50% cytotoxic dose
Vero Cells
Pharmacology
MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
Hepatitis B Surface Antigens
fungi
POMs, Polyoxometalates
medicine.disease
digestive system diseases
HBV, hepatitis B virus
3TC, nucleoside analog lamivudine
DNA, Viral
INF-α, interferon alpha
Subjects
Details
- Language :
- English
- ISSN :
- 18729096 and 01663542
- Volume :
- 93
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Antiviral Research
- Accession number :
- edsair.doi.dedup.....af82afcdc90851a17390f390ca8ee259