Back to Search
Start Over
Sleep deprivation induces changes in immunity in Trichinella spiralis-infected rats
- Source :
- International Journal of Biological Sciences
- Publication Year :
- 2015
-
Abstract
- Sleep is considered an important predictor of immunity. A lack of sleep may reduce immunity, which increases susceptibility to any type of infection. Moreover, sleep deprivation in humans produces changes in both, the percent of circulating immune cells (T cells and NK cells) and cytokine levels (IL-1, IFNγ, TNΦ-αα, IL-6 and IL-17). The aim of our study was to investigate whether sleep deprivation produces deregulation on immune variables during the immune response generated against the helminth parasite Trichinella spiralis. Because sleep deprivation is stressful per se, we designed another experiments to compared stress alone (consisting in movement restriction and single housing) with sleep deprivation, in both control (uninfected) and experimental (infected) rats. Our results demonstrate that the sleep deprivation and stress have a differential effect in mesenteric lymph nodes (MLN) and spleen. In uninfected rats sleep deprivation alone produces an increase in natural killer cells (NK+) and B cells (CD45+), accompanied by a decrease in cytotoxic T cells (CD3+CD8+) in spleen; while, in MLN, produces only an increase in natural killer cells (NK+). Both, SD and stress, produce an increased percentage of total T cells (CD3+) in spleen. In the MLN both are also associated to an increase in cytotoxic T cells (CD3+CD8+) and B cells (CD45+). In the spleens of parasitized rats, cell populations did not change. In spleens of both, sleep-deprived and stressed infected rats, we observed an increase in B cells (CD45+). In infected rats, sleep deprivation alone produced an increase in NK cells (NK+). In mesenteric node cell populations of parasitized rats, we observed a decrease in NK cells and an increase in T helper (CD4+) cells in both SD and stressed rats. Rats that were only subjected to stress showed a decrease in B cells (CD45+). These findings suggest that the immune response generated against infection caused by T. spiralis is affected when the sleep pattern is disrupted. These results support the notion that sleep is a fundamental process for an adequate and strong immune response generated against this parasite.
- Subjects :
- Male
medicine.medical_specialty
medicine.medical_treatment
Trichinella spiralis
Spleen
Applied Microbiology and Biotechnology
immune response
Immunophenotyping
Immune system
Immunity
Internal medicine
medicine
Cytotoxic T cell
Animals
Rats, Wistar
Molecular Biology
Ecology, Evolution, Behavior and Systematics
biology
Trichinellosis
Cell Biology
biology.organism_classification
sleep deprivation
Lymphocyte Subsets
Rats
Sleep deprivation
medicine.anatomical_structure
Endocrinology
Cytokine
Immunology
parasite
Cytokines
medicine.symptom
CD8
Developmental Biology
Research Paper
Subjects
Details
- ISSN :
- 14492288
- Volume :
- 11
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- International journal of biological sciences
- Accession number :
- edsair.doi.dedup.....af987d20b51dfb7bacf7b406362e24c8