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Quantitative SERS Detection of Dopamine in Cerebrospinal Fluid by Dual-Recognition-Induced Hot Spot Generation
- Source :
- ACS Applied Materials & Interfaces. 10:15388-15394
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- Reliable profiling of the extracellular dopamine (DA) concentration in the central nervous system is essential for a deep understanding of its biological and pathological functions. However, quantitative determination of this neurotransmitter remains a challenge because of the extremely low concentration of DA in the cerebrospinal fluid (CSF) of patients. Herein, on the basis of the specific recognition of boronate toward diol and N-hydroxysuccinimide ester toward the amine group, a simple and highly sensitive strategy was presented for DA detection by using surface-enhanced Raman scattering (SERS) spectroscopy as a signal readout. This was realized by first immobilizing 3,3'-dithiodipropionic acid di( N-hydroxysuccinimide ester) on gold thin film surfaces to capture DA, followed by introducing 3-mercaptophenylboronic acid (3-MPBA)-functionalized silver nanoparticles to generate numerous plasmonic "hot spots" with the nanoparticle-on-mirror geometry. Such a dual-recognition mechanism not only avoids complicated bioelement-based manipulations but also efficiently decreases the background signal. With the direct use of the recognition probe 3-MPBA as a Raman reporter, the "signal-on" SERS method was employed to quantify the concentration of DA from 1 pM to 1 μM with a detection limit of 0.3 pM. Moreover, our dual-recognition-directed SERS assay exhibited a high resistance to cerebral interference and was successfully applied to monitoring of DA in CSF samples of patients.
- Subjects :
- Silver
Materials science
Dopamine
Diol
Metal Nanoparticles
02 engineering and technology
Spectrum Analysis, Raman
010402 general chemistry
01 natural sciences
Silver nanoparticle
symbols.namesake
chemistry.chemical_compound
Cerebrospinal fluid
medicine
General Materials Science
021001 nanoscience & nanotechnology
Quantitative determination
0104 chemical sciences
chemistry
symbols
Biophysics
Amine gas treating
Gold
0210 nano-technology
Raman scattering
Extracellular dopamine
medicine.drug
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....afaf890e5444f1c07b1ad28d7f5a01b2
- Full Text :
- https://doi.org/10.1021/acsami.8b01063