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iTRAQ protein profile analysis of developmental dynamics in soybean [Glycine max (L.) Merr.] leaves
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 9, p e0181910 (2017)
- Publication Year :
- 2016
-
Abstract
- Zao5241 is an elite soybean [Glycine max (L.) Merr.] line and backbone parent. In this study, we employed iTRAQ to analyze the proteomes and protein expression profiles of Zao5241 during leaf development. We identified 1,245 proteins in all experiments, of which only 45 had been previously annotated. Among overlapping proteins between three biological replicates, 598 proteins with 2 unique peptides identified were reliably quantified. The protein datasets were classified into 36 GO functional terms, and the photosynthesis term was most significantly enriched. A total of 113 proteins were defined as being differentially expressed during leaf development; 41 proteins were found to be differently expressed between two and four week old leaves, and 84 proteins were found to be differently expressed between two and six week old leaves, respectively. Cluster analysis of the data revealed dynamic proteomes. Proteins annotated as electron carrier activity were greatly enriched in the peak expression profiles, and photosynthesis proteins were negatively modulated along the whole time course. This dataset will serve as the foundation for a systems biology approach to understanding photosynthetic development.
- Subjects :
- 0301 basic medicine
Proteomics
Leaves
Proteomes
Protein Expression
lcsh:Medicine
Plant Science
resource
Biochemistry
stress
Gene Expression Regulation, Plant
Tandem Mass Spectrometry
Gene expression
Cluster Analysis
Photosynthesis
lcsh:Science
genes
database
technologies
Plant Proteins
Regulation of gene expression
Plant Growth and Development
Multidisciplinary
Plant Biochemistry
Plant Anatomy
Applied Mathematics
Simulation and Modeling
Agriculture
Proteome
Physical Sciences
reveals
Algorithms
Research Article
kyoto encyclopedia
Plant Development
Crops
Biology
Research and Analysis Methods
03 medical and health sciences
Clustering Algorithms
proteomics
Botany
Gene Expression and Vector Techniques
Molecular Biology Techniques
Gene
Molecular Biology
Molecular Biology Assays and Analysis Techniques
lcsh:R
Electron carrier activity
Biology and Life Sciences
Proteins
Leaf Development
Plant Leaves
030104 developmental biology
Glycine
identification
lcsh:Q
Soybeans
genomes
Soybean
Mathematics
Crop Science
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....b00057312e829912355b61aa8dbdb233