Long Non-coding RNA T-UCstem1 Controls Progenitor Proliferation and Neurogenesis in the Postnatal Mouse Olfactory Bulb through Interaction with miR-9

Summary Neural stem cell populations generate a wide spectrum of neuronal and glial cell types in a highly ordered fashion. MicroRNAs are essential regulators of this process. T-UCstem1 is a long non-coding RNA containing an ultraconserved element, and in vitro analyses in pluripotent stem cells provided evidence that it regulates the balance between proliferation and differentiation. Here we investigate the in vivo function of T-UCstem1. We show that T-UCstem1 is expressed in the forebrain neurogenic lineage that generates interneurons for the postnatal olfactory bulb. Gain of function in neural stem cells increased progenitor proliferation at the expense of neuron production, whereas knockdown had the opposite effect. This regulatory function is mediated by its interaction with miR-9-3p and miR-9-5p. Based thereon, we propose a mechanistic model for the role of T-UCstem1 in the dynamic regulation of neural progenitor proliferation during neurogenesis.
Graphical Abstract
Highlights • T-UCstem1 is expressed in the SVZ-RMS-OB neurogenic system • T-UCstem1 controls proliferation of neuronal progenitors in the subventricular zone • T-UCstem1 regulates neuron addition to the olfactory bulb • T-UCstem1 acts through miR-9-3p and miR-9-5p
In this study, Fico, Beclin, and colleagues show that the long non-coding RNA T-UCstem1 is expressed in the forebrain neurogenic lineage generating interneurons for the postnatal olfactory bulb. Increased T-UCstem1 levels in neural stem cells induced progenitor proliferation at the expense of neuron production, whereas its downregulation caused the opposite effect. This regulatory function is mediated by its interaction with miR-9.