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Mouse Models of Genetically Altered Peroxiredoxin 6
- Source :
- Antioxidants, Antioxidants, Vol 8, Iss 4, p 77 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Peroxiredoxin 6 (Prdx6) has been shown to have three enzymatic activities: peroxidase, phospholipase A2 (PLA2) and acyl transferase. The peroxidase activity is unusual, as it is capable of reducing phospholipid hydroperoxides (as well as hydrogen peroxide and short chain organic peroxides). Knockout and overexpressing mice have been produced that demonstrate the effect that eliminating or overproducing Prdx6 has on the animals’ physiology. In addition, mutations in various amino acids of Prdx6 have been identified that interfere with different enzymatic functions as well as protein transport. These mutations were originally characterized biochemically; subsequently, several knock-in mouse strains have been produced, each containing one mutation. These mice include the S32T knock-in that affects protein transport, the C47S knock-in that inactivates the peroxidase enzymatic activity, the D140A knock-in that inactivates the PLA2 enzymatic activity and the H26A knock-in that inactivates the peroxidase and blocks binding to phospholipids. This review summarizes the properties of these mice based upon studies conducted with the knockout, overexpressing and knock-in mice and the effect of the genetic changes on the biochemistry and physiology of these mice. The availability of these mice is also briefly discussed.
- Subjects :
- Physiology
Clinical Biochemistry
peroxidase
Review
Phospholipase
medicine.disease_cause
Biochemistry
03 medical and health sciences
0302 clinical medicine
Phospholipase A2
phospholipid hydroperoxide
knock-in mouse
medicine
Molecular Biology
030304 developmental biology
chemistry.chemical_classification
0303 health sciences
Mutation
phospholipase A2
biology
Chemistry
lcsh:RM1-950
lipid peroxidation
Cell Biology
Transport protein
Amino acid
lcsh:Therapeutics. Pharmacology
Enzyme
membrane repair
030220 oncology & carcinogenesis
Knockout mouse
biology.protein
knockout mouse
Peroxidase
Subjects
Details
- ISSN :
- 20763921
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Antioxidants
- Accession number :
- edsair.doi.dedup.....b003d6afb3bc6425c487720f70f45758
- Full Text :
- https://doi.org/10.3390/antiox8040077