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Puerarin Inhibits oxLDL-Induced Macrophage Activation and Foam Cell Formation in Human THP1 Macrophage
- Source :
- BioMed Research International, Vol 2015 (2015), BioMed Research International
- Publication Year :
- 2015
- Publisher :
- Hindawi Limited, 2015.
-
Abstract
- Puerarin, an isoflavone derived from Kudzu roots, has been widely used for treatment of cardiovascular and cerebral vascular diseases in China and other Asian countries. However, the underlying mechanisms are largely unknown. The present study investigated whether puerarin inhibited atherogenic lipid oxLDL-mediated macrophage activation and foam cell formation in human THP1 macrophage. Treatment with oxLDL significantly increased the mRNA expression of proinflammatory cytokines tumor necrosis factorα(TNFα, 160%) and interleukin (IL) 1β(13 fold) accompanied by upregulation of toll-like receptor 4 (TLR4, 165%) and the ratio of phospho-IκBα/IκBαin THP1 macrophage. Puerarin dose-dependently prevented an increase in oxLDL-induced proinflammatory gene expression with downregulation of TLR4 and the ratio of phospho-IκBα/IκBα. Furthermore, puerarin prevented oxLDL-mediated lipid deposition and foam cell formation associated with downregulation of scavenger receptor CD36. Flow cytometry analysis showed that puerarin reduced the number of early apoptotic cells of macrophages induced by oxLDL. Our results show that puerarin has anti-inflammatory and antiatherogenic effects in vitro; the underlying mechanisms may involve the inhibition of TLR4/NFκB pathway and downregulation of CD36 expression. The results from the present study provide scientific evidence and may expand our armamentarium to use puerarin for prevention and treatment of cardiovascular and atherosclerotic diseases.
- Subjects :
- Article Subject
CD36
lcsh:Medicine
Pharmacology
General Biochemistry, Genetics and Molecular Biology
Proinflammatory cytokine
Cell Line
chemistry.chemical_compound
Downregulation and upregulation
Puerarin
Humans
Drug Interactions
Scavenger receptor
Cells, Cultured
Foam cell
General Immunology and Microbiology
biology
Dose-Response Relationship, Drug
Chemistry
Macrophages
lcsh:R
General Medicine
Macrophage Activation
Isoflavones
Lipoproteins, LDL
IκBα
Immunology
TLR4
biology.protein
lipids (amino acids, peptides, and proteins)
Inflammation Mediators
Foam Cells
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2015
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....b01711eb6dedf191718251c41264a513