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Remodeling Chromatin Induces Z-DNA Conformation Detected through Fourier Transform Infrared Spectroscopy
- Source :
- Analytical chemistry. 92(21)
- Publication Year :
- 2020
-
Abstract
- The SWI/SNF complex is a highly conserved chromatin remodeling complex and can hydrolyze ATP by its catalytic subunit BRG1 or BRM to reconstruct the chromatin. To investigate whether this ATP-dependent chromatin remodeling could affect the DNA conformation, we therefore regulated (knocked down or overexpressed) BRG1/BRM in the cells and applied Fourier transform infrared (FTIR) spectroscopy to probe DNA conformational changes. As a result, we found that BRG1/BRM was indeed associated with the DNA conformational changes, in which knockdown of BRG1/BRM reduced Z-DNA conformation, while overexpression of BRG1/BRM enhanced Z-DNA conformation. This Z-DNA conformational transformation was also verified using the Z-DNA-binding proteins. Therefore, this work has provided a direct analytical tool to probe Z-DNA transformation upon ATP-dependent chromatin remodeling.
- Subjects :
- animal structures
Protein subunit
010402 general chemistry
01 natural sciences
Chromatin remodeling
Analytical Chemistry
Z-DNA
chemistry.chemical_compound
Adenosine Triphosphate
Cell Line, Tumor
Spectroscopy, Fourier Transform Infrared
DNA, Z-Form
Humans
Fourier transform infrared spectroscopy
Gene knockdown
Chemistry
010401 analytical chemistry
DNA Helicases
Nuclear Proteins
Chromatin Assembly and Disassembly
0104 chemical sciences
Chromatin
Transformation (genetics)
Gene Knockdown Techniques
Biophysics
Nucleic Acid Conformation
DNA
Transcription Factors
Subjects
Details
- ISSN :
- 15206882
- Volume :
- 92
- Issue :
- 21
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....b01ec62d580986e068a9975c6446636e