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Flavonolignans Inhibit IL1-β-Induced Cross-Talk between Blood Platelets and Leukocytes
- Source :
- Nutrients, Vol 9, Iss 9, p 1022 (2017), Nutrients, Nutrients; Volume 9; Issue 9; Pages: 1022
- Publication Year :
- 2017
- Publisher :
- MDPI AG, 2017.
-
Abstract
- Interleukin-1 beta (IL-1β)—the most potent pro-inflammatory is responsible for a broad spectrum of immune and inflammatory responses, it induces T-cell and B-cell activation and consequently the synthesis of other pro-inflammatory cytokines (such as IFN-γ and TNF). IL-1β induces the formation of blood platelet-leukocyte aggregates (PLAs), which suggests that IL-1β significantly affects the cross-talk between blood platelets and the immune response system, leading to coronary thrombosis. The aim of our study is to investigate the effect of flavonolignans (silybin, silychristin and silydianin) on the IL-1β-induced interaction between platelets and leukocytes, as well as on the expression and the secretion of pro-inflammatory factors. Whole blood samples were pre-incubated with commercially available flavonolignans (silybin, silychristin and silydianin) in a concentration range of 10–100 µM (30 min, 37 °C). Next, samples were activated by IL-1β for 1 h. Blood platelet-leukocyte aggregates were detected by using the double-labeled flow cytometry (CD61/CD45). The level of produced cytokines was estimated via the ELISA immunoenzymatic method. IFN-γ and TNF gene expression was evaluated using Real Time PCR with TaqMan arrays. We observed that in a dose-dependent manner, silybin and silychristin inhibit the IL-1β-induced formation of blood platelet-leukocyte aggregates in whole blood samples, as well as the production of pro-inflammatory cytokines—IL-2, TNF, INF-α, and INF-γ. Additionally, these two flavonolignans abolished the IL-1β-induced expression of mRNA for IFN-γ and TNF. Our current results demonstrate that flavonolignans can be novel compounds used in the prevention of cardiovascular diseases with dual-use action as antiplatelet and anti-inflammatory agents.
- Subjects :
- 0301 basic medicine
Blood Platelets
Interleukin-1beta
Anti-Inflammatory Agents
lcsh:TX341-641
030204 cardiovascular system & hematology
Biology
Pharmacology
interleukin 1
Article
silybin
Flow cytometry
Flavonolignans
03 medical and health sciences
0302 clinical medicine
Immune system
flavonolignans
medicine
Leukocytes
Humans
Platelet
Cells, Cultured
Whole blood
anti-inflammatory
Nutrition and Dietetics
medicine.diagnostic_test
silychristin
Interleukin
030104 developmental biology
Biochemistry
Cytokines
Tumor necrosis factor alpha
CD61
lcsh:Nutrition. Foods and food supply
Platelet Aggregation Inhibitors
Food Science
Silymarin
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 9
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....b02f51ad3bd6b2de61377168bf1c137f