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Development of nanostructured bioanodes containing dendrimers and dehydrogenases enzymes for application in ethanol biofuel cells
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2010
-
Abstract
- This paper describes the use of the electrostatic layer-by-layer (LbL) technique for the preparation of bioanodes with potential application in ethanol/O2 biofuel cells. More specifically, the LbL technique was employed for immobilization of dehydrogenase enzymes and polyamidoamine (PAMAM) dendrimers onto carbon paper support. Both mono (anchoring only the enzyme alcohol dehydrogenase, ADH) and bi-enzymatic (anchoring both ADH and aldehyde dehydrogenase, AldDH) systems were tested. The amount of ADH deposited onto the Toray® paper was 95 ng cm−2 per bilayer. Kinetic studies revealed that the LbL technique enables better control of enzyme disposition on the bioanode, as compared with the results obtained with the bioanodes prepared by the passive adsorption technique. The power density values achieved for the mono-enzymatic system as a function of the enzyme load ranged from 0.02 to 0.063 mW cm−2 for the bioanode containing 36 ADH bilayers. The bioanodes containing a gas diffusion layer (GDL) displayed enhanced performance, but their mechanical stability must be improved. The bi-enzymatic system generated a power density of 0.12 mW cm−2. In conclusion, the LbL technique is a very attractive approach for enzyme immobilization onto carbon platform, since it enables strict control of enzyme disposition on the bioanode surface with very low enzyme consumption.
- Subjects :
- Paper
Dendrimers
Immobilized enzyme
Bioelectric Energy Sources
Static Electricity
Biomedical Engineering
Biophysics
Dehydrogenase
Enzyme anchoring
chemistry.chemical_compound
Adsorption
Dendrimer
Electrochemistry
Organic chemistry
Nanotechnology
Alcohol dehydrogenase
Ethanol
Layer by layer
biology
Bioanode
Bilayer
Alcohol Dehydrogenase
General Medicine
Aldehyde Dehydrogenase
Enzymes, Immobilized
Combinatorial chemistry
ENZIMAS
Carbon
Nanostructures
Kinetics
Ethanol biofuel cell
chemistry
biology.protein
Microelectrodes
Oxidation-Reduction
Biotechnology
Subjects
Details
- ISSN :
- 18734235
- Volume :
- 26
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Biosensorsbioelectronics
- Accession number :
- edsair.doi.dedup.....b039c072ae56a160fdb8c8258cf30bc2