Back to Search
Start Over
Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR
- Source :
- Journal of Virological Methods
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was used for generation of standard RNAs. The detection limit of the assay was 1 × 10 2 RNA copies and standard curve displayed a linear range from 1 × 10 2 to 1 × 10 9 copies and a good reproducibility. The assay was then applied to determine the mRNA loads in the tissues of dogs naturally infected by CPV-2. mRNA was detected in a variety of tissues, including the central nervous system.
- Subjects :
- Parvovirus, Canine
RNA Splicing
mRNA
viruses
CPV-2
Sensitivity and Specificity
Article
Parvoviridae Infections
Dogs
Virology
TaqMan
Animals
Infectious virus
Dog Diseases
RNA, Messenger
Parvoviridae
Messenger RNA
biology
Reverse Transcriptase Polymerase Chain Reaction
Parvovirus
Canine parvovirus
Brain
RNA
Viral Load
biology.organism_classification
Molecular biology
Real-time RT-PCR
Reverse transcription polymerase chain reaction
Real-time polymerase chain reaction
RNA, Viral
Subjects
Details
- ISSN :
- 01660934
- Volume :
- 146
- Database :
- OpenAIRE
- Journal :
- Journal of Virological Methods
- Accession number :
- edsair.doi.dedup.....b03ff490badc1ec597cd2c6e49c09027