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Retrospective analysis on confirmation rates for referred positive rotavirus samples in England, 2016 to 2017: implications for diagnosis and surveillance
Retrospective analysis on confirmation rates for referred positive rotavirus samples in England, 2016 to 2017: implications for diagnosis and surveillance
- Source :
- Eurosurveillance
- Publication Year :
- 2020
- Publisher :
- European Centre for Disease Control and Prevention (ECDC), 2020.
-
Abstract
- Background Rapid diagnostic tests are commonly used by hospital laboratories in England to detect rotavirus (RV), and results are used to inform clinical management and support national surveillance of the infant rotavirus immunisation programme since 2013. In 2017, the Public Health England (PHE) national reference laboratory for enteric viruses observed that the presence of RV could not be confirmed by PCR in a proportion of RV-positive samples referred for confirmatory detection. Aim We aimed to compare the positivity rate of detection methods used by hospital laboratories with the PHE confirmatory test rate. Methods Rotavirus specimens testing positive at local hospital laboratories were re-tested at the PHE national reference laboratory using a PCR test. Confirmatory results were compared to original results from the PHE laboratory information management system. Results Hospital laboratories screened 70.1% (2,608/3,721) of RV samples using immunochromatographic assay (IC) or rapid tests, 15.5% (578/3,721) using enzyme immunoassays (EIA) and 14.4% (535/3,721) using PCR. Overall, 1,011/3,721 (27.2%) locally RV-positive samples referred to PHE in 2016 and 2017 failed RV detection using the PHE reference laboratory PCR test. Confirmation rates were 66.9% (1,746/2,608) for the IC tests, 87.4% (505/578) for the EIA and 86.4% (465/535) for the PCR assays. Seasonal confirmation rate discrepancies were also evident for IC tests. Conclusions This report highlights high false positive rates with the most commonly used RV screening tests and emphasises the importance of implementing verified confirmatory tests for RV detections. This has implications for clinical diagnosis and national surveillance.
- Subjects :
- Rotavirus
antibiotic resistance
IMPACT
diagnosis
Epidemiology
Pcr assay
SUSCEPTIBILITY
molecular methods
medicine.disease_cause
THERAPY
VACCINE INTRODUCTION
antibiotic use
rapid test
GENOMIC EPIDEMIOLOGY
Retrospective analysis
Public Health Surveillance
Infectious Diseases
England
surveillance
GROUP-A ROTAVIRUS
NEISSERIA-GONORRHOEAE
Enzyme immunoassays
Life Sciences & Biomedicine
0605 Microbiology
medicine.medical_specialty
TRANSMISSION
Point-of-care testing
Rotavirus Infections
1117 Public Health and Health Services
modelling
AGE
multidrug resistance
Pcr test
ACUTE GASTROENTERITIS
Virology
Internal medicine
medicine
Humans
antimicrobial resistance
gonorrhoea
sexually transmitted infections
Retrospective Studies
Science & Technology
business.industry
Research
screening
Public Health, Environmental and Occupational Health
Infant
Retrospective cohort study
bacterial infections
point-of-care tests
business
1199 Other Medical and Health Sciences
Subjects
Details
- ISSN :
- 15607917
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Eurosurveillance
- Accession number :
- edsair.doi.dedup.....b0436f5e5380eaff75ad95229ad0770e
- Full Text :
- https://doi.org/10.2807/1560-7917.es.2020.25.43.1900375