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Gene Structures and Expression Profiles of Three Human KCND (Kv4) Potassium Channels Mediating A-Type Currents ITO and ISA
- Source :
- Genomics. 64:144-154
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- The four known members of the KCND/Kv4 channel family encode voltage-gated potassium channels. Recent studies provide evidence that members of the Kv4 channel family are responsible for native, rapidly inactivating (A-type) currents described in heart (I(TO)) and neurons (I(SA)). In this study, we cloned the human KCND1 cDNA, localized the KCND1 gene to chromosome Xp11.23-p11.3, and determined the genomic structure and tissue-specific expression of the KCND1, KCND2, and KCND3 genes, respectively. The open reading frame of Kv4. 1 is 1941 nucleotides long, predicting a protein of 647 amino acids. The deduced protein sequence of Kv4.1 shows an overall identity of 60% with Kv4.2 and Kv4.3L and corresponds to the common structure of voltage-gated potassium channels. KCND1-specific transcripts were detectable in human brain, heart, liver, kidney, thyroid gland, and pancreas, as revealed by Northern blot and RT-PCR experiments. The comparison of the expression patterns of the known Kv4 family members shows subtype specificity with significant overlaps. The KCND gene structures exhibit an evolutionarily conserved exon pattern with a large first exon containing the intracellular N-terminus and the putative membrane-spanning regions S1 to S5, as well as part of the pore region. The KCND3 gene contains an additional exon of 57 bp, which is not present in the other two KCND genes and gives rise to the C-terminal splice KCND3L variant with an insertion of 19 amino acids.
- Subjects :
- DNA, Complementary
Potassium Channels
Transcription, Genetic
Molecular Sequence Data
Gene Expression
Biology
Polymerase Chain Reaction
Homology (biology)
03 medical and health sciences
Exon
0302 clinical medicine
Complementary DNA
Gene expression
Genetics
Humans
Tissue Distribution
RNA, Messenger
Northern blot
Cloning, Molecular
Gene
Gene Library
030304 developmental biology
0303 health sciences
Genome
Sequence Homology, Amino Acid
Electric Conductivity
Nucleic acid sequence
Chromosome Mapping
Exons
Molecular biology
Introns
Open reading frame
Shal Potassium Channels
Potassium Channels, Voltage-Gated
cardiovascular system
Ion Channel Gating
Sequence Alignment
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 08887543
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Genomics
- Accession number :
- edsair.doi.dedup.....b0532be320ccb0fb1e3a4f26afc8cd2a