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Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification
- Source :
- Journal of Virological Methods
- Publication Year :
- 2008
- Publisher :
- Elsevier B.V., 2008.
-
Abstract
- A method of loop-mediated isothermal amplification (LAMP) was employed to develop a rapid and simple detection system for porcine circovirus type 2 (PCV2). The amplification could be finished in 60 min under isothermal condition at 64 degrees C by employing a set of four primers targeting the cap gene of PCV2. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of five copies per tube of purified PCV2 genomic DNA. No cross-reactivity was observed from the samples of other related viruses including porcine circovirus type 1 (PCV1), porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV). The detection rate of PCV2 LAMP for 86 clinical samples was 96.5% and appeared greater than that of the PCR method. The LAMP assay reported can provide a rapid yet simple test of PCV2 suitable for laboratory diagnosis and pen-side detection due to ease of operation and the requirement of only a regular water bath or heat block for the reaction.
- Subjects :
- Circovirus
Porcine parvovirus
Loop-mediated isothermal amplification (LAMP)
Time Factors
Swine
viruses
animal diseases
Porcine circovirus type 2 (PCV2)
Loop-mediated isothermal amplification
Sensitivity and Specificity
Virus
Article
Sensitivity
Virology
Animals
Circoviridae Infections
DNA Primers
Detection limit
Swine Diseases
biology
Temperature
virus diseases
biology.organism_classification
Porcine reproductive and respiratory syndrome virus
Molecular biology
Porcine circovirus
Detection
Specificity
Circoviridae
Nucleic Acid Amplification Techniques
Subjects
Details
- Language :
- English
- ISSN :
- 18790984 and 01660934
- Volume :
- 149
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Virological Methods
- Accession number :
- edsair.doi.dedup.....b0657178a388e13d6e8b27d7b6c98c79