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The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice
- Source :
- Journal of Visualized Experiments : JoVE
- Publication Year :
- 2014
- Publisher :
- MyJove Corporation, 2014.
-
Abstract
- Neuropathic pain arises as a consequence of a lesion or a disease affecting the somatosensory system. This syndrome results from maladaptive changes in injured sensory neurons and along the entire nociceptive pathway within the central nervous system. It is usually chronic and challenging to treat. In order to study neuropathic pain and its treatments, different models have been developed in rodents. These models derive from known etiologies, thus reproducing peripheral nerve injuries, central injuries, and metabolic-, infectious- or chemotherapy-related neuropathies. Murine models of peripheral nerve injury often target the sciatic nerve which is easy to access and allows nociceptive tests on the hind paw. These models rely on a compression and/or a section. Here, the detailed surgery procedure for the "cuff model" of neuropathic pain in mice is described. In this model, a cuff of PE-20 polyethylene tubing of standardized length (2 mm) is unilaterally implanted around the main branch of the sciatic nerve. It induces a long-lasting mechanical allodynia, i.e., a nociceptive response to a normally non-nociceptive stimulus that can be evaluated by using von Frey filaments. Besides the detailed surgery and testing procedures, the interest of this model for the study of neuropathic pain mechanism, for the study of neuropathic pain sensory and anxiodepressive aspects, and for the study of neuropathic pain treatments are also discussed.
- Subjects :
- Male
General Chemical Engineering
Central nervous system
Somatosensory system
General Biochemistry, Genetics and Molecular Biology
Lesion
Mice
Medicine
Animals
pain
mouse
sciatic
allodynia
neuropathic pain
Issue 89
model
General Immunology and Microbiology
business.industry
General Neuroscience
Sciatic Nerve
Mice, Inbred C57BL
Disease Models, Animal
Nociception
Allodynia
medicine.anatomical_structure
Hyperalgesia
Anesthesia
Peripheral nerve injury
Neuropathic pain
Neuralgia
Sciatic nerve
medicine.symptom
cuff
Sciatic Neuropathy
business
Neuroscience
von Frey
Subjects
Details
- Language :
- English
- ISSN :
- 1940087X
- Issue :
- 89
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments : JoVE
- Accession number :
- edsair.doi.dedup.....b06c75e8ba1767d7dae7eeb1eaff6ddb