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Metabolic regulation of the lysosomal cofactor bis(monoacylglycero)phosphate in mice
- Source :
- Journal of Lipid Research, Vol 61, Iss 7, Pp 995-1003 (2020), Journal of Lipid Research
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Bis(monoacylglycero)phosphate (BMP), also known as lysobisphosphatidic acid, is a phospholipid that promotes lipid sorting in late endosomes/lysosomes by activating lipid hydrolases and lipid transfer proteins. Changes in the cellular BMP content therefore reflect an altered metabolic activity of the endolysosomal system. Surprisingly, little is known about the physiological regulation of BMP. In this study, we investigated the effects of nutritional and metabolic factors on BMP profiles of whole tissues and parenchymal and nonparenchymal cells. Tissue samples were obtained from fed, fasted, 2 h refed, and insulin-treated mice, as well as from mice housed at 5°C, 22°C, or 30°C. These tissues exhibited distinct BMP profiles that were regulated by the nutritional state in a tissue-specific manner. Insulin treatment was not sufficient to mimic refeeding-induced changes in tissue BMP levels, indicating that BMP metabolism is regulated by other hormonal or nutritional factors. Tissue fractionation experiments revealed that fasting drastically elevates BMP levels in hepatocytes and pancreatic cells. Furthermore, we observed that the BMP content in brown adipose tissue strongly depends on housing temperatures. In conclusion, our observations suggest that BMP concentrations adapt to the metabolic state in a tissue- and cell-type-specific manner in mice. Drastic changes observed in hepatocytes, pancreatic cells, and brown adipocytes suggest that BMP plays a role in the functional adaption to nutrient starvation and ambient temperature.
- Subjects :
- 0301 basic medicine
insulin
Endosome
Phospholipid
Adipose tissue
Endosomes
QD415-436
030204 cardiovascular system & hematology
liver
Biochemistry
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Lysosome
lipid metabolism
Brown adipose tissue
medicine
Animals
pancreas
Research Articles
phospholipids
nutritional state
Chemistry
Macrophages
Lipid metabolism
Cell Biology
Metabolism
adipose tissue
Cell biology
030104 developmental biology
medicine.anatomical_structure
embryonic structures
lysosome
Monoglycerides
Lysophospholipids
Lysosomes
body temperature
Plant lipid transfer proteins
Subjects
Details
- ISSN :
- 00222275
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Journal of Lipid Research
- Accession number :
- edsair.doi.dedup.....b0b4e1ee9b0eafecf4c3f5b5055ec03c