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Peptidomics Analysis Reveals Peptide PDCryab1 Inhibits Doxorubicin-Induced Cardiotoxicity
- Source :
- Oxidative Medicine and Cellular Longevity, Oxidative Medicine and Cellular Longevity, Vol 2020 (2020)
- Publication Year :
- 2020
-
Abstract
- Doxorubicin (DOX) is limited due to dose-dependent cardiotoxicity. Peptidomics is an emerging field of proteomics that has attracted much attention because it can be used to study the composition and content of endogenous peptides in various organisms. Endogenous peptides participate in various biological processes and are important sources of candidates for drug development. To explore peptide changes related to DOX-induced cardiotoxicity and to find peptides with cardioprotective function, we compared the expression profiles of peptides in the hearts of DOX-treated and control mice by mass spectrometry. The results showed that 236 differential peptides were identified upon DOX treatment, of which 22 were upregulated and 214 were downregulated. Next, we predicted that 31 peptides may have cardioprotective function by conducting bioinformatics analysis on the domains of each precursor protein, the predicted score of peptide biological activity, and the correlation of each peptide with cardiac events. Finally, we verified that a peptide (SPFYLRPPSF) from Cryab can inhibit cardiomyocyte apoptosis, reduce the production of reactive oxygen species, improve cardiac function, and ameliorate myocardial fibrosis in vitro and vivo. In conclusion, our results showed that the expression profiles of peptides in cardiac tissue change significantly upon DOX treatment and that these differentially expressed peptides have potential cardioprotective functions. Our study suggests a new direction for the treatment of DOX-induced cardiotoxicity.
- Subjects :
- 0301 basic medicine
Male
Proteomics
Aging
Article Subject
Cell Survival
Down-Regulation
Peptide
Endogeny
030204 cardiovascular system & hematology
Pharmacology
Biochemistry
Mass Spectrometry
Ventricular Function, Left
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Animals
Doxorubicin
Myocytes, Cardiac
Protein Interaction Maps
Chromatography, High Pressure Liquid
chemistry.chemical_classification
Reactive oxygen species
Cardiotoxicity
QH573-671
Chemistry
Myocardium
Biological activity
Heart
Cell Biology
General Medicine
In vitro
Rats
Up-Regulation
Mice, Inbred C57BL
030104 developmental biology
Cytology
Peptides
Reactive Oxygen Species
medicine.drug
Research Article
Subjects
Details
- ISSN :
- 19420994
- Volume :
- 2020
- Database :
- OpenAIRE
- Journal :
- Oxidative medicine and cellular longevity
- Accession number :
- edsair.doi.dedup.....b0dce1d81e9e653510b8139014930f0f