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Tetrachloromethane-Degrading Bacterial Enrichment Cultures and Isolates from a Contaminated Aquifer
- Source :
- Microorganisms, Vol 3, Iss 3, Pp 327-343 (2015), Microorganisms, Volume 3, Issue 3, Pages 327-343
- Publication Year :
- 2015
- Publisher :
- MDPI AG, 2015.
-
Abstract
- The prokaryotic community of a groundwater aquifer exposed to high concentrations of tetrachloromethane (CCl4) for more than three decades was followed by terminal restriction fragment length polymorphism (T-RFLP) during pump-and-treat remediation at the contamination source. Bacterial enrichments and isolates were obtained under selective anoxic conditions, and degraded 10 mg·L−1 CCl4, with less than 10% transient formation of chloroform. Dichloromethane and chloromethane were not detected. Several tetrachloromethane-degrading strains were isolated from these enrichments, including bacteria from the Klebsiella and Clostridium genera closely related to previously described CCl4 degrading bacteria, and strain TM1, assigned to the genus Pelosinus, for which this property was not yet described. Pelosinus sp. TM1, an oxygen-tolerant, Gram-positive bacterium with strictly anaerobic metabolism, excreted a thermostable metabolite into the culture medium that allowed extracellular CCl4 transformation. As estimated by T-RFLP, phylotypes of CCl4-degrading enrichment cultures represented less than 7%, and archaeal and Pelosinus strains less than 0.5% of the total prokaryotic groundwater community.
- Subjects :
- Microbiology (medical)
Klebsiella
Metabolite
Microbiology
Article
chemistry.chemical_compound
Clostridium
tetrachloromethane
Virology
C1 compound biodegradation
lcsh:QH301-705.5
Pelosinus sp. TM1
Phylotype
biology
Strain (chemistry)
bacterial communities
biology.organism_classification
Anoxic waters
Terminal restriction fragment length polymorphism
lcsh:Biology (General)
chemistry
co-metabolism
bacterial enrichment cultures
carbon tetrachloride
chlorinated solvents
Bacteria
Subjects
Details
- ISSN :
- 20762607
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....b0f40dcce4c2054dedd38f8f32ad3185