Back to Search
Start Over
Epigallocatechin-3-gallate Ameliorates Seawater Aspiration-Induced Acute Lung Injury via Regulating Inflammatory Cytokines and Inhibiting JAK/STAT1 Pathway in Rats
- Source :
- Mediators of Inflammation, Mediators of Inflammation, Vol 2014 (2014)
- Publication Year :
- 2014
- Publisher :
- Hindawi Limited, 2014.
-
Abstract
- Signal transducers and activators of transcriptions 1 (STAT1) play an important role in the inflammation process of acute lung injury (ALI). Epigallocatechin-3-gallate (EGCG) exhibits a specific and strong anti-STAT1 activity. Therefore, our study is to explore whether EGCG pretreatment can ameliorate seawater aspiration-induced ALI and its possible mechanisms. We detected the arterial partial pressure of oxygen, lung wet/dry weight ratios, protein content in bronchoalveolar lavage fluid, and the histopathologic and ultrastructure staining of the lung. The levels of IL-1, TNF-α, and IL-10 and the total and the phosphorylated protein level of STAT1, JAK1, and JAK2 were assessed in vitro and in vivo. The results showed that EGCG pretreatment significantly improved hypoxemia and histopathologic changes, alleviated pulmonary edema and lung vascular leak, reduced the production of TNF-αand IL-1, and increased the production of IL-10 in seawater aspiration-induced ALI rats. EGCG also prevented the seawater aspiration-induced increase of TNF-αand IL-1 and decrease of IL-10 in NR8383 cell line. Moreover, EGCG pretreatment reduced the total and the phosphorylated protein level of STAT1in vivoandin vitroand reduced the phosphorylated protein level of JAK1 and JAK2. The present study demonstrates that EGCG ameliorates seawater aspiration-induced ALI via regulating inflammatory cytokines and inhibiting JAK/STAT1 pathway in rats.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Article Subject
Partial Pressure
Acute Lung Injury
Immunology
Lung injury
Pharmacology
Biology
Catechin
Proinflammatory cytokine
Rats, Sprague-Dawley
In vivo
lcsh:Pathology
medicine
Animals
Arterial Pressure
Seawater
Phosphorylation
Lung
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
Macrophages
Respiratory Aspiration
food and beverages
Janus Kinase 1
Cell Biology
Janus Kinase 2
Pulmonary edema
medicine.disease
Interleukin-10
Rats
respiratory tract diseases
Oxygen
Interleukin 10
STAT1 Transcription Factor
Bronchoalveolar lavage
medicine.anatomical_structure
Cytokines
Tumor necrosis factor alpha
Bronchoalveolar Lavage Fluid
lcsh:RB1-214
Interleukin-1
Signal Transduction
Research Article
Subjects
Details
- ISSN :
- 14661861 and 09629351
- Volume :
- 2014
- Database :
- OpenAIRE
- Journal :
- Mediators of Inflammation
- Accession number :
- edsair.doi.dedup.....b115e073c3f27c452616946f579ae6a5