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Heterogeneity of Moraxella isolates found in the nasal cavities of piglets
- Source :
- BMC Veterinary Research, Vol 16, Iss 1, Pp 1-12 (2020), IRTA Pubpro. Open Digital Archive, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), BMC Veterinary Research, Dipòsit Digital de Documents de la UAB, Universitat Autònoma de Barcelona
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Background Previous studies have shown that the genus Moraxella is commonly present in the nasal microbiota of swine. Results In this study, 51 isolates of Moraxella were obtained from nasal swabs from 3 to 4 week old piglets, which represented 26 different fingerprintings by enterobacterial repetitive intergenic consensus (ERIC)-PCR. Whole 16S rRNA gene sequencing allowed the identification at species level of the Moraxella spp. isolates. The majority of the field strains were identified as Moraxella pluranimalium, but Moraxella porci was also detected. In addition, a cluster of 7 strains did not group with any described Moraxella species, probably representing a new species. Subsequent phenotypic characterization indicated that strains of Moraxella pluranimalium were mainly sensitive to serum complement, while the cluster representing the putative new species was highly resistant. Biofilm formation capacity was very variable among the Moraxella spp. isolates, while adherence to epithelial cell lines was similar among selected strains. Additionally, variability was also observed in the association of selected strains to porcine alveolar macrophages. Antimicrobial tests evidenced the existence of multidrug-resistance in the strains. Conclusions In summary, phenotypic characterization revealed heterogeneity among Moraxella strains from the nasal cavity of piglets. Strains with pathogenic potential were detected as well as those that may be commensal members of the nasal microbiota. However, the role of Moraxella in porcine diseases and health should be further evaluated.
- Subjects :
- Nasal cavity
Swine
Virulence
Cell Line
Microbiology
03 medical and health sciences
Intergenic region
Anti-Infective Agents
Drug Resistance, Multiple, Bacterial
RNA, Ribosomal, 16S
Macrophages, Alveolar
otorhinolaryngologic diseases
medicine
Animals
Humans
Moraxella
030304 developmental biology
0303 health sciences
lcsh:Veterinary medicine
General Veterinary
biology
Nasal microbiota
030306 microbiology
Biofilm
General Medicine
Antimicrobial
biology.organism_classification
Phenotype
medicine.anatomical_structure
Bacterial diversity
A549 Cells
Nasal Swab
Biofilms
bacteria
lcsh:SF600-1100
Nasal Cavity
Research Article
Subjects
Details
- ISSN :
- 17466148
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- BMC Veterinary Research
- Accession number :
- edsair.doi.dedup.....b116acc4e21d6fce1d4a5658215d1a7b