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A possible role of cIAP2 in Helicobacter pylori-associated gastric cancer
- Source :
- Cancer letters. 313(2)
- Publication Year :
- 2011
-
Abstract
- Cellular inhibitor of apoptosis protein 2 (cIAP2) is a member of the IAP family and is over-expressed in most cancer tissues. In this study, we investigated the role cIAP2 in Helicobacter pylori (HP) related gastric carcinogenesis. We measured the expression of cIAP2 at mRNA and protein levels in a panel of gastric cancer cell lines and human gastric cancer tissues by semi-quantitative reverse transcriptase PCR (RT-PCR), quantitative real time PCR (qPCR), immunoblotting, and immunohistochemistry. The effects of cIAP2 down-regulation on gastric cell proliferation and apoptosis were detected by standard WST-1 assay and flow cytometry, respectively. Infection of gastric mucosa by HP enhances the expression of cIAP2 in mouse gastric tissues. Over 70% of human gastric cancer tissues express higher amount of cIAP2. Well-differentiated gastric cancer cells express more cIAP2 than moderately- and poorly-differentiated gastric cancer cells. Knocking down of cIAP2 in SGC-7901 cells results in a 30% decrease in cell proliferation, a 20% increase in apoptosis and delayed migration. Thus, cIAP2 may play an important role in HP-induced gastric carcinogenesis, and it may serve as a potential target for gastric cancer therapy.
- Subjects :
- Male
Cancer Research
Ubiquitin-Protein Ligases
Down-Regulation
Apoptosis
Inhibitor of apoptosis
medicine.disease_cause
Inhibitor of Apoptosis Proteins
Mice
Cell Movement
Stomach Neoplasms
Cell Line, Tumor
Gastric mucosa
medicine
Animals
Humans
Cell Proliferation
biology
Helicobacter pylori
Reverse Transcriptase Polymerase Chain Reaction
digestive, oral, and skin physiology
Cancer
medicine.disease
biology.organism_classification
Molecular biology
digestive system diseases
Baculoviral IAP Repeat-Containing 3 Protein
Reverse transcription polymerase chain reaction
medicine.anatomical_structure
Oncology
Gastric Mucosa
Cancer cell
RNA Interference
Carcinogenesis
Subjects
Details
- ISSN :
- 18727980
- Volume :
- 313
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cancer letters
- Accession number :
- edsair.doi.dedup.....b11ccb0506531a4256eb49b6100d480a