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Transcriptomic Characterization Reveals Attributes of High Influenza Virus Productivity in MDCK Cells
- Source :
- Viruses, Vol 13, Iss 2200, p 2200 (2021), Viruses, Volume 13, Issue 11
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- The Madin–Darby Canine Kidney (MDCK) cell line is among the most commonly used cell lines for the production of influenza virus vaccines. As cell culture-based manufacturing is poised to replace egg-based processes, increasing virus production is of paramount importance. To shed light on factors affecting virus productivity, we isolated a subline, H1, which had twice the influenza virus A (IAV) productivity of the parent (P) through cell cloning, and characterized H1 and P in detail on both physical and molecular levels. Transcriptome analysis revealed that within a few hours after IAV infection, viral mRNAs constituted over one fifth of total mRNA, with several viral genes more highly expressed in H1 than P. Functional analysis of the transcriptome dynamics showed that H1 and P responded similarly to IAV infection, and were both subjected to host shutoff and inflammatory responses. Importantly, H1 was more active in translation and RNA processing intrinsically and after infection. Furthermore, H1 had more subdued inflammatory and antiviral responses. Taken together, we postulate that the high productivity of IAV hinges on the balance between suppression of host functions to divert cellular resources and the sustaining of sufficient activities for virus replication. Mechanistic insights into virus productivity can facilitate the process optimization and cell line engineering for advancing influenza vaccine manufacturing.
- Subjects :
- Influenza vaccine
Apoptosis
Biology
Virus Replication
Microbiology
Virus
Article
influenza virus
Cell Line
Madin Darby Canine Kidney Cells
Transcriptome
Dogs
host shutoff
Virology
Influenza, Human
Animals
Humans
Messenger RNA
Host (biology)
Hemagglutination
MDCK cells
Translation (biology)
Orthomyxoviridae
QR1-502
Infectious Diseases
Viral replication
Cell culture
Influenza A virus
Influenza Vaccines
transcriptome
anti-viral responses
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Volume :
- 13
- Issue :
- 2200
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....b140abfe559b01a7d53350ee2b2824b5