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Splenectomy in zebrafish: a new model for immune thrombocytopenia
- Source :
- Platelets
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- In humans, splenectomy is performed to treat many clinical disorders, including immune thrombocytopenia. However, the incidence of splenectomies for immune thrombocytopenia as a therapeutic has significantly declined over the past decade due to the availability of new therapies. Infection and sepsis as a result of splenectomies are well documented, but other long-term effects are not well characterized. Evidence suggests that persons who have had a prior splenectomy may be at an increased risk of vascular conditions. Also, elevated levels of cell-derived microparticles appear to contribute to an increased risk of thrombosis and cardiovascular disease. However, in vivo studies on the increased levels of microparticles following splenectomy are limited. In order to understand the effects of splenectomies, we developed a protocol for splenectomy in adult zebrafish. After anesthesia, the spleen was removed under a stereomicroscope after making an incision on the ventral side of the fish. The spleen was removed by pulling with forceps. The incision was closed by Vetbond tissue glue. Blood collected from both splenectomized zebrafish and those that underwent sham surgeries was immunolabeled with polyclonal antisera against αIIb, followed by flow cytometry. We observed elevated levels of thrombocytes and their microparticles in splenectomized zebrafish. Finally, by injecting αIIb antibody intravenously into zebrafish, we found the thrombocyte counts decreased, suggesting the fish developed immune thrombocytopenia like conditions, which were then reversed by splenectomy. In summary, the model developed here should be useful to study molecular changes due to splenectomy. Also, the zebrafish will be useful in modeling treatment of immune thrombocytopenia like conditions.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
medicine.medical_treatment
Splenectomy
Spleen
030204 cardiovascular system & hematology
Article
Sepsis
03 medical and health sciences
0302 clinical medicine
In vivo
Animals
Humans
Medicine
Platelet
Zebrafish
Purpura, Thrombocytopenic, Idiopathic
biology
business.industry
Hematology
General Medicine
medicine.disease
biology.organism_classification
Thrombosis
030104 developmental biology
medicine.anatomical_structure
biology.protein
Antibody
business
Subjects
Details
- ISSN :
- 13691635 and 09537104
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Platelets
- Accession number :
- edsair.doi.dedup.....b14432c8a51f5f2238b5ad20e5d1ffa2