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Bacterial community structure and functional arrAgene diversity associated with arsenic reductionand release in an industrially contaminated soil

Authors :
Francis Garrido
Corinne Leyval
Catherine Joulian
Marianne Quéméneur
Patrick Billard
Dominique Breeze
Michel Jauzein
Institut méditerranéen d'océanologie (MIO)
Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)
Bureau de Recherches Géologiques et Minières (BRGM) (BRGM)
Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)
Institut Ecologie et Environnement (INEE)
Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo)
Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)
Ecole Nationale Supérieure des Mines de Nancy (ENSMN)
Institut Mines-Télécom [Paris] (IMT)-Université de Lorraine (UL)
ANR-10-LABX-0100,VOLTAIRE,Geofluids and Volatil elements – Earth, Atmosphere, Interfaces – Resources and Environment(2010)
European Project: 505428,GOCE
Institut méditerranéen d'océanologie ( MIO )
Institut de Recherche pour le Développement ( IRD ) -Aix Marseille Université ( AMU ) -Université de Toulon ( UTLN ) -Centre National de la Recherche Scientifique ( CNRS )
Bureau de Recherches Géologiques et Minières (BRGM) ( BRGM )
Laboratoire Interdisciplinaire des Environnements Continentaux ( LIEC )
Université de Lorraine ( UL ) -Centre National de la Recherche Scientifique ( CNRS )
Ecole Nationale Supérieure des Mines de Nancy ( ENSMN )
Institut Mines-Télécom [Paris]-Université de Lorraine ( UL )
ANR-10-LABX-100-01/10-LABX-0100,VOLTAIRE,Geofluids and Volatil elements – Earth, Atmosphere, Interfaces – Resources and Environment ( 2010 )
European Project : 505428,GOCE
Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo)
Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE)
Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Toulon (UTLN)
Source :
Geomicrobiology Journal, Geomicrobiology Journal, 2016, 33 (10), pp.839-849. ⟨10.1080/01490451.2015.1118167⟩, Geomicrobiology Journal, Taylor & Francis, 2016, 33 (10), pp.839-849. 〈10.1080/01490451.2015.1118167〉, Geomicrobiology Journal, Taylor & Francis, 2016, 33 (10), pp.839-849. ⟨10.1080/01490451.2015.1118167⟩
Publication Year :
2016
Publisher :
HAL CCSD, 2016.

Abstract

International audience; This study aimed at evaluating potential arsenic (As) mobility in an industrially contaminatedsoil (64 mg As kg-1) of the Meuse River basin, and at identifying key bacterial groups that drive soil As dynamics. Both speciation and release of As from this soil was followed under anaerobicconditions using a laboratory batch experiment. In the presence of exogenous carbon sources,AsV initially present in the soil matrix and/or adsorbed on synthetic hydrous ferric oxides wassolubilized and mainly reduced into AsIII by indigenous soil microfora. After a one-monthincubation period in these biotic conditions, AsIII accounted for 80-85% of the total dissolved Asand more than 60% of the solid-phase As. Bacterial community structure (i.e. 16S rDNA-basedCE-SSCP profiles) changed with incubation time and As amendment. The detection of distantlyrelated arsenate respiratory reductase genes (arrA), as functional markers of AsV-respirers,indicates that novel dissimilatory AsV-reducing bacteria may be involved in Asbiotransformation and mobility in anoxic soils. Since As and iron were concomitantly released, acrucial role of indirect As-mobilizing bacteria on As behavior was also revealed. Our results show that the majority of As within the soil matrix was bioavailable and bioaccessible forheterotrophic AsV reduction to AsIII, which may increase As toxicity and mobility in thecontaminated soils.

Details

Language :
English
ISSN :
01490451 and 15210529
Database :
OpenAIRE
Journal :
Geomicrobiology Journal, Geomicrobiology Journal, 2016, 33 (10), pp.839-849. ⟨10.1080/01490451.2015.1118167⟩, Geomicrobiology Journal, Taylor & Francis, 2016, 33 (10), pp.839-849. 〈10.1080/01490451.2015.1118167〉, Geomicrobiology Journal, Taylor & Francis, 2016, 33 (10), pp.839-849. ⟨10.1080/01490451.2015.1118167⟩
Accession number :
edsair.doi.dedup.....b160c45d5f0ec565ad7984f0f8358c90
Full Text :
https://doi.org/10.1080/01490451.2015.1118167⟩