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Impact of individual intravenous iron preparations on the differentiation of monocytes towards macrophages and dendritic cells
- Source :
- Nephrology Dialysis Transplantation
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Background Treatment of iron deficiency with intravenous (i.v.) iron is a first-line strategy to improve anaemia of chronic kidney disease. Previous in vitro experiments demonstrated that different i.v. iron preparations inhibit differentiation of haematopoietic stem cells to monocytes, but their effect on monocyte differentiation to macrophages and mature dendritic cells (mDCs) has not been assessed. We investigated substance-specific effects of iron sucrose (IS), sodium ferric gluconate (SFG), ferric carboxymaltose (FCM) and iron isomaltoside 1000 (IIM) on monocytic differentiation to M1/M2 macrophages and mDCs. Methods Via flow cytometry and microRNA (miRNA) expression analysis, we morphologically and functionally characterized monocyte differentiation to M1/M2 macrophages and mDCs after monocyte stimulation with IS, SFG, FCM and IIM (0.133, 0.266 and 0.533 mg/mL, respectively). To assess potential clinical implications, we compared monocytic phagocytosis capacity in dialysis patients who received either 500 mg IS or IIM. Results Phenotypically, IS and SFG dysregulated the expression of macrophage (e.g. CD40, CD163) and mDC (e.g. CD1c, CD141) surface markers. Functionally, IS and SFG impaired macrophage phagocytosis capacity. Phenotypic and functional alterations were less pronounced with FCM, and virtually absent with IIM. In miRNA expression analysis of mDCs, IS dysregulated miRNAs such as miR-146b-5p and miR-155-5p, which are linked to Toll-like receptor and mitogen-activated protein kinase signalling pathways. In vivo, IS reduced monocytic phagocytosis capacity within 1 h after infusion, while IIM did not. Conclusions This study demonstrates that less stable i.v. iron preparations specifically affect monocyte differentiation towards macrophages and mDCs.
- Subjects :
- 0301 basic medicine
030232 urology & nephrology
Pharmacology
Disaccharides
Iron sucrose
Ferric Compounds
Monocytes
Glucaric Acid
03 medical and health sciences
0302 clinical medicine
Basic Science
Phagocytosis
Iron Isomaltoside 1000
CKD
Humans
Medicine
Macrophage
Maltose
Ferric Oxide, Saccharated
iron therapy
Transplantation
Anemia, Iron-Deficiency
business.industry
Macrophages
Cell Differentiation
Original Articles
Dendritic Cells
Iron deficiency
immune deficiency
medicine.disease
In vitro
MicroRNAs
Haematopoiesis
030104 developmental biology
Nephrology
Case-Control Studies
Monocyte differentiation
Injections, Intravenous
Immunology
Hematinics
Stem cell
business
Iron Compounds
medicine.drug
Subjects
Details
- ISSN :
- 14602385 and 09310509
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Nephrology Dialysis Transplantation
- Accession number :
- edsair.doi.dedup.....b1625ba6a6f17593be6f406a27a17ff4