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Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa

Authors :
Qingbing Zha
Qiaolang Zeng
Xin Yan
Zhijie Li
Jiansu Chen
Mengyuan Xie
Shanshan Shi
Yonglong Guo
Jun Zhang
Peiyuan Wang
Shiwei Liu
Yunxia Xue
Quan Yu
Shibo Tang
Hongjie Ma
Zekai Cui
Source :
Stem Cell Research, Vol 29, Iss, Pp 139-142 (2018)
Publication Year :
2018

Abstract

We have established an induced pluripotent stem (iPS) cell line using urine-derived cells from a 27-year-old male patient with retinitis pigmentosa associated with point mutations in the USH2A gene. Feeder-free culture conditions and the integration-free CytoTuneâ„¢-iPS 2.0 Sendai Reprogramming Kit were used.

Details

ISSN :
18767753
Volume :
29
Database :
OpenAIRE
Journal :
Stem cell research
Accession number :
edsair.doi.dedup.....b1740f1be69db26c723ccb793f1bf057