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Requirement for DARPP-32 in progesterone-facilitated sexual receptivity in female rats and mice
Requirement for DARPP-32 in progesterone-facilitated sexual receptivity in female rats and mice
- Source :
- Science (New York, N.Y.). 287(5455)
- Publication Year :
- 2000
-
Abstract
- DARPP-32, a dopamine- and adenosine 3′,5′-monophosphate (cAMP)–regulated phosphoprotein (32 kilodaltons in size), is an obligate intermediate in progesterone (P)–facilitated sexual receptivity in female rats and mice. The facilitative effect of P on sexual receptivity in female rats was blocked by antisense oligonucleotides to DARPP-32. Homozygous mice carrying a null mutation for the DARPP-32 gene exhibited minimal levels of P-facilitated sexual receptivity when compared to their wild-type littermates. P significantly increased hypothalamic cAMP levels and cAMP-dependent protein kinase activity. These increases were not inhibited by a D 1 subclass dopamine receptor antagonist. P also enhanced phosphorylation of DARPP-32 on threonine 34 in the hypothalamus of mice. DARPP-32 activation is thus an obligatory step in progestin receptor regulation of sexual receptivity in rats and mice.
- Subjects :
- Male
medicine.medical_specialty
Dopamine and cAMP-Regulated Phosphoprotein 32
Serotonin
Dopamine
Posture
Hypothalamus
Nerve Tissue Proteins
Biology
Rats, Sprague-Dawley
Mice
Sexual Behavior, Animal
Internal medicine
medicine
Cyclic AMP
Animals
Phosphorylation
Protein kinase A
Receptor
Progesterone
Injections, Intraventricular
Mice, Knockout
Multidisciplinary
Proteins
Oligonucleotides, Antisense
Phosphoproteins
Adenosine
Cyclic AMP-Dependent Protein Kinases
Rats
Mice, Inbred C57BL
Endocrinology
Dopamine receptor
Phosphoprotein
Dopamine Agonists
Female
2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
Signal transduction
Receptors, Progesterone
medicine.drug
Signal Transduction
Subjects
Details
- ISSN :
- 00368075
- Volume :
- 287
- Issue :
- 5455
- Database :
- OpenAIRE
- Journal :
- Science (New York, N.Y.)
- Accession number :
- edsair.doi.dedup.....b1d840e65e045d8ce919d0b24d5ef937