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Immunological comparison of pregnant Dahl salt-sensitive and Sprague-Dawley rats commonly used to model characteristics of preeclampsia
- Source :
- Am J Physiol Regul Integr Comp Physiol
- Publication Year :
- 2021
- Publisher :
- American Physiological Society, 2021.
-
Abstract
- The pregnant Dahl salt-sensitive (S) rat is an established preclinical model of superimposed spontaneous preeclampsia characterized by exacerbated hypertension, increased urinary protein excretion, and increased fetal demise. Because of the underlying immune system dysfunction present in preeclamptic pregnancies in humans, we hypothesized that the pregnant Dahl S rat would also have an altered immune status. Immune system activation was assessed during late pregnancy in the Dahl S model and compared with healthy pregnant Sprague-Dawley (SD) rats subjected to either a sham procedure or a procedure to reduce uterine perfusion pressure (RUPP). Circulating immunoglobulin and cytokine levels were measured by enzyme-linked immunosorbent assay (ELISA) and Milliplex bead assay, respectively, and percentages of circulating, splenic, and placental immune cells were determined using flow cytometry. The pregnant Dahl S rat exhibited an increase in CD4+ T cells, and specifically TNFα+CD4+ T cells, in the spleen compared with virgin Dahl S rats. The Dahl also had increased neutrophils and decreased B cells in the peripheral blood as compared with Dahl virgin rats. SD rats that received the RUPP procedure had increases in circulating monocytes and increased IFN-ɣ+CD4+ splenic T cells. Together these findings suggest that dysregulated T cell activity is an important factor in both the pregnant Dahl S rats and SD rats after the RUPP procedure.
- Subjects :
- CD4-Positive T-Lymphocytes
0301 basic medicine
Urinary protein
medicine.medical_specialty
Neutrophils
Physiology
Placenta
medicine.medical_treatment
Immunoglobulins
Gestational Age
030204 cardiovascular system & hematology
Preeclampsia
Rats, Sprague-Dawley
Excretion
03 medical and health sciences
0302 clinical medicine
Immune system
Pre-Eclampsia
Pregnancy
Physiology (medical)
Internal medicine
medicine
Sprague dawley rats
Animals
Dahl salt sensitive
B-Lymphocytes
Rats, Inbred Dahl
biology
business.industry
medicine.disease
Disease Models, Animal
Phenotype
030104 developmental biology
Endocrinology
Cytokine
biology.protein
Cytokines
Female
Antibody
business
Spleen
Research Article
Subjects
Details
- ISSN :
- 15221490 and 03636119
- Volume :
- 321
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Accession number :
- edsair.doi.dedup.....b20f42c82ceebc2941e50d8197739fdb