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Heterologous resistance to superinfection by louping ill virus persistently infected cell cultures
- Source :
- Archives of Virology. 125:251-259
- Publication Year :
- 1992
- Publisher :
- Springer Science and Business Media LLC, 1992.
-
Abstract
- Louping ill virus, a tick-borne arbovirus readily established a persistent infection in porcine kidney (PS) cells after initially inducing minor cytopathic changes. Nucleotide sequence analysis of the envelope glycoprotein of the viral RNA recovered from the persistently infected cells showed no changes as compared with the virus used to establish persistent infections. More than 80 per cent of the cells contained virus specific antigen when analysed by indirect immunofluorescence microscopy. This persistently infected cell line resisted superinfection with either homologous or most heterologous flaviviruses. However, the yellow fever French neurotropic virus (YF FNV) multiplied in the persistently infected cells and evidence of dual infections in these cells was obtained using specific monoclonal antibodies in double labelling immunofluorescence tests. The relevance of these observations is discussed in the light of other evidence that tick-borne viruses can survive for long periods in wild animal species.
- Subjects :
- viruses
Molecular Sequence Data
DNA, Single-Stranded
Heterologous
medicine.disease_cause
Immunofluorescence
Arbovirus
Virus
Cell Line
Encephalitis Viruses, Tick-Borne
Microbiology
Antigen
Virology
Viral Interference
medicine
Animals
Neurotropic virus
Base Sequence
biology
medicine.diagnostic_test
Flavivirus
General Medicine
medicine.disease
biology.organism_classification
Superinfection
Subjects
Details
- ISSN :
- 14328798 and 03048608
- Volume :
- 125
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....b25a441f91eb78ca1228edfc12d2a6b7