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Fluid Shear Stress Increases Osteocyte and Inhibits Osteoclasts via Downregulating Receptor-Activator of Nuclear Factor κB (RANK)/Osteoprotegerin Expression in Myeloma Microenvironment
- Source :
- Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
- Publication Year :
- 2019
- Publisher :
- International Scientific Information, Inc., 2019.
-
Abstract
- BACKGROUND The aim of this study was to determine the effects of myeloma cells exposed to fluid shear stress on osteocytes and osteoclasts, and clarify the potential underlying mechanisms. MATERIAL AND METHODS A flow and a non-flow model were established using a flow fluid chamber. The myeloma cell line U266 and murine osteocytic MLO-Y4 cells were cultured in vitro. The osteocytes and osteoclasts were examined under a microscope. Osteoclasts were stained for tartrate-resistant acid phosphatase (TRAP) activity. RANKL and osteoprotegerin (OPG) gene expression were detected using reverse transcription-quantitative polymerase chain reaction. RESULTS Compared with the controls, Y4 cells cultured with U266 culture supernatant showed altered morphology, fewer osteocytes, increased RANKL gene expression, a higher RANKL/OPG gene ratio, and a greater number of TRAP-positive osteoclasts (P
- Subjects :
- musculoskeletal diseases
Osteoclasts
Osteoclast proliferation
030204 cardiovascular system & hematology
Osteocytes
Mice
03 medical and health sciences
0302 clinical medicine
Osteoprotegerin
Lab/In Vitro Research
Cell Line, Tumor
Gene expression
Tumor Microenvironment
medicine
Animals
Humans
Multiple myeloma
Receptor Activator of Nuclear Factor-kappa B
biology
Activator (genetics)
Chemistry
RANK Ligand
General Medicine
medicine.disease
Biomechanical Phenomena
medicine.anatomical_structure
Gene Expression Regulation
Cell culture
RANKL
030220 oncology & carcinogenesis
Osteocyte
Hydrodynamics
Cancer research
biology.protein
Stress, Mechanical
Multiple Myeloma
Shear Strength
Subjects
Details
- ISSN :
- 16433750
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Medical Science Monitor
- Accession number :
- edsair.doi.dedup.....b25ba242d2134bdffd4faab94477d0b1