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Serum human telomerase reverse transcriptase messenger RNA as a novel tumor marker for hepatocellular carcinoma
- Source :
- Clinical cancer research : an official journal of the American Association for Cancer Research. 11(9)
- Publication Year :
- 2005
-
Abstract
- Purpose: We previously reported the usefulness of a qualified highly sensitive detection method for human telomerase reverse transcriptase (hTERT) mRNA in serum with 89.7% sensitivity for hepatocellular carcinoma (HCC). In this study, we developed a quantitative detection method for serum hTERT mRNA and examined the clinical significance in HCC diagnosis. Experimental Background: In 64 patients with HCC, 20 with liver cirrhosis, 20 with chronic hepatitis, and 50 healthy individuals, we measured serum hTERT mRNA by using the newly developed real-time quantitative reverse transcription-PCR with SYBR Green I. We examined its sensitivity and specificity in HCC diagnosis, clinical significance in comparison with other tumor markers, and its correlations with the clinical variables by using multivariate analyses. Results: Serum hTERT mRNA showed higher values in patients with HCC than those with chronic liver diseases. hTERT mRNA expression was shown to be independently correlated with clinical variables such as tumor size, number, and degree of differentiation (P < 0.001, each). The sensitivity/specificity of hTERT mRNA and alpha;-fetoprotein (AFP) mRNA in HCC diagnosis were 88.2%/70.0% for hTERT and 71.6%/67.5% for AFP, respectively. hTERT mRNA proved to be superior to AFP mRNA, AFP, and des-γ-carboxy prothrombin in HCC diagnosis. Furthermore, hTERT mRNA in serum was associated with that in HCC tissue. Conclusions: The usefulness of hTERT mRNA expression in HCC diagnosis and its superiority to conventional tumor markers were shown. Therefore, serum hTERT mRNA is a novel and available marker for HCC diagnosis.
- Subjects :
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Telomerase
Cirrhosis
Carcinoma, Hepatocellular
Biology
Sensitivity and Specificity
medicine
Biomarkers, Tumor
Humans
Clinical significance
Telomerase reverse transcriptase
RNA, Messenger
neoplasms
Tumor marker
Aged
Aged, 80 and over
Messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
Liver Neoplasms
Middle Aged
medicine.disease
digestive system diseases
Reverse transcriptase
DNA-Binding Proteins
enzymes and coenzymes (carbohydrates)
Oncology
Hepatocellular carcinoma
embryonic structures
Cancer research
Female
Subjects
Details
- ISSN :
- 10780432
- Volume :
- 11
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Clinical cancer research : an official journal of the American Association for Cancer Research
- Accession number :
- edsair.doi.dedup.....b2aedc7d1a95a3b5636aa39e78a49692