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Transcription factor YY1 promotes adipogenesis via inhibiting CHOP-10 expression
- Source :
- Biochemical and Biophysical Research Communications. 375:496-500
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- CHOP-10, a dominant-negative member of the C/EBP family of transcription factors, is initially expressed by growth-arrested preadipocytes and sequesters/inactivates C/EBPbeta through heterodimerization with its leucine zipper during 3T3-L1 preadipocyte differentiation. Our previous studies indicated that, FBS leads to the down-regulation of CHOP-10 expression after induction, and releasing C/EBPbeta from inhibitory constraint, allowing the transactivation of C/EBPalpha and PPARgamma genes, transcription factors required for terminal adipocyte differentiation. In the present study, we reported that FBS induced the expression of YY1, which bound to CHOP-10 promoter via two adjacent YY1-binding sites, suppressing its expression. The knock-down of YY1 expression with YY1 siRNA increased the expression of CHOP-10, inhibiting adipocyte differentiation. IGF-1, a growth factor present in greater concentration in FBS, independently induced the expression of YY1, and contributed to the down-regulation of CHOP-10 during the adipocyte differentiation program. Our studies suggested that YY1 can be a new adipocyte differentiation stimulator.
- Subjects :
- Serum
Leucine zipper
medicine.medical_treatment
Biophysics
Biology
Biochemistry
Mice
chemistry.chemical_compound
Transactivation
3T3-L1 Cells
Adipocyte
medicine
Animals
Insulin-Like Growth Factor I
RNA, Small Interfering
Promoter Regions, Genetic
Molecular Biology
Transcription factor
YY1 Transcription Factor
Regulation of gene expression
ATF3
Adipogenesis
Growth factor
Cell Biology
Cell biology
Gene Expression Regulation
chemistry
embryonic structures
Cancer research
Cattle
biological phenomena, cell phenomena, and immunity
Transcription Factor CHOP
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 375
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....b2b838109d6362cc51d44c5c0ec769a3