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Detection of antineutrophil cytoplasmic antibodies (ANCAs)
- Source :
- Annals of the Rheumatic Diseases, 76(4), 647-653. BMJ Publishing Group
- Publication Year :
- 2017
-
Abstract
- ObjectiveThis multicentre study was performed to evaluate the diagnostic accuracy of a wide spectrum of novel technologies nowadays available for detection of myeloperoxidase (MPO) and proteinase 3 (PR3)-antineutrophil cytoplasmic antibodies (ANCAs).MethodsSera (obtained at the time of diagnosis) from 251 patients with ANCA-associated vasculitis (AAV), including granulomatosis with polyangiitis and microscopic polyangiitis, and from 924 disease controls were tested for the presence of cytoplasmic pattern/perinuclear pattern and atypical ANCA (A-ANCA) by indirect immunofluorescence (IIF) (at two sites) and for the presence of PR3-ANCA and MPO-ANCA by eight different immunoassays.ResultsThe area under the curve (AUC) of the receiver operating characteristic curve to discriminate AAV from controls was 0.923 (95% CI 0.902 to 0.944) and 0.843 (95% CI 0.814 to 0.871) for the two IIF methods. For the antigen-specific immunoassays, the AUC varied between 0.936 (95% CI 0.912 to 0.960) and 0.959 (95% CI 0.941 to 0.976), except for one immunoassay for which the AUC was 0.919 (95% CI 0.892 to 0.945).ConclusionsOur comparison of various ANCA detection methods showed (i) large variability between the two IIF methods tested and (ii) a high diagnostic performance of PR3-ANCA and MPO-ANCA by immunoassay to discriminate AAV from disease controls. Consequently, dual IIF/antigen-specific immunoassay testing of each sample is not necessary for maximal diagnostic accuracy. These results indicate that the current international consensus on ANCA testing for AAV needs revision.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Myeloblastin
Immunology
Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis
SYSTEMIC VASCULITIS
CAPTURE ELISA
General Biochemistry, Genetics and Molecular Biology
Antibodies, Antineutrophil Cytoplasmic
03 medical and health sciences
0302 clinical medicine
WEGENERS-GRANULOMATOSIS
Rheumatology
Proteinase 3
medicine
Immunology and Allergy
Fluorescent Antibody Technique, Indirect
PROTEINASE-3
Anti-neutrophil cytoplasmic antibody
Peroxidase
030203 arthritis & rheumatology
Immunoassay
LINKED-IMMUNOSORBENT-ASSAY
medicine.diagnostic_test
business.industry
Area under the curve
IIf
medicine.disease
MPO-ANCA
030104 developmental biology
ROC Curve
Area Under Curve
Case-Control Studies
AUTOANTIBODIES
business
Microscopic polyangiitis
Granulomatosis with polyangiitis
FOLLOW-UP
CRESCENTIC GLOMERULONEPHRITIS
Systemic vasculitis
INFLAMMATORY-BOWEL-DISEASE
Subjects
Details
- Language :
- English
- ISSN :
- 00034967
- Volume :
- 76
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Annals of the Rheumatic Diseases
- Accession number :
- edsair.doi.dedup.....b2c0c966e4aff69d793f9d7153c74931