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Preparation of a Fucoidan-Grafted Hyaluronan Composite Hydrogel for the Induction of Osteoblast Differentiation in Osteoblast-Like Cells
- Source :
- Materials, Volume 14, Issue 5, Materials, Vol 14, Iss 1168, p 1168 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- A suitable bone substitute is necessary in bone regenerative medicine. Hyaluronan (HA) has excellent biocompatibility and biodegradability and is widely used in tissue engineering. Additionally, research on fucoidan (Fu), a fucose- and sulfate-rich polysaccharide from brown seaweed, for the promotion of bone osteogenic differentiation has increased exponentially. In this study, HA and Fu were functionalized by grafting methacrylic groups onto the backbone of the chain. Methacrylate-hyaluronan (MHA) and methacrylate-fucoidan (MFu) were characterized by FTIR and 1H NMR spectroscopy to confirm functionalization. The degrees of methacrylation (DMs) of MHA and MFu were 9.2% and 98.6%, respectively. Furthermore, we evaluated the mechanical properties of the hydrogels formed from mixtures of photo-crosslinkable MHA (1%) with varying concentrations of MFu (0%, 0.5%, and 1%). There were no changes in the hardness values of the hydrogels, but the elastic modulus decreased upon the addition of MFu, and these mechanical properties were not significantly different with or without preosteoblastic MG63 cell culture for up to 28 days. Furthermore, the cell morphologies and viabilities were not significantly different after culture with the MHA, MHA-MFu0.5, or MHA-MFu1.0 hydrogels, but the specific activity and mineralization of alkaline phosphatase (ALP) were significantly higher in the MHA-MFu1.0 hydrogel group compared to the other hydrogels. Hence, MHA-MFu composite hydrogels are potential bone graft materials that can provide a flexible structure and favorable niche for inducing bone osteogenic differentiation.
- Subjects :
- Biocompatibility
0206 medical engineering
02 engineering and technology
lcsh:Technology
Fucose
Article
hyaluronan
chemistry.chemical_compound
Tissue engineering
fucoidan
medicine
General Materials Science
lcsh:Microscopy
lcsh:QC120-168.85
lcsh:QH201-278.5
lcsh:T
Fucoidan
technology, industry, and agriculture
Osteoblast
021001 nanoscience & nanotechnology
020601 biomedical engineering
medicine.anatomical_structure
chemistry
lcsh:TA1-2040
Self-healing hydrogels
Biophysics
osteoblast
Surface modification
Alkaline phosphatase
lcsh:Descriptive and experimental mechanics
lcsh:Electrical engineering. Electronics. Nuclear engineering
hydrogel
lcsh:Engineering (General). Civil engineering (General)
0210 nano-technology
lcsh:TK1-9971
Subjects
Details
- Language :
- English
- ISSN :
- 19961944
- Volume :
- 14
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Materials
- Accession number :
- edsair.doi.dedup.....b2d9a8925adb457c195a22c5dbdad604