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Mis-localization of Arp2 mRNA impairs persistence of directional cell migration
- Source :
- Experimental cell research. 317(6)
- Publication Year :
- 2010
-
Abstract
- Arp2/3 complex is an actin polymerization nucleator and localized in the leading protrusions of migrating cells. It has been unclear how this complex is targeted to the protrusions and whether its localization is functionally important. We previously demonstrated that mRNAs encoding for the subunits of the complex were localized in the protrusions of fibroblasts, suggesting a mechanism to target the complex to the protrusions. We here present data demonstrating the importance of Arp2/3 complex mRNA localization in directional cell migration. Using a novel mechanism by which Dia1 mRNA is targeted to the perinuclear endoplasmic reticulum, we re-directed the mRNA encoding Arp2, a subunit of the Arp2/3 complex, to the perinuclear region in fibroblasts. Knockdown of Arp2 alone caused dramatic reduction of the complex and resulted in narrow protrusions, increased random cell migration speed and loss of directionality. Rescue with a protrusion-localizing Arp2 mRNA restored normal cell migration behavior, whereas rescue with a mis-localizing Arp2 mRNA failed to restore speed and directionality. These results demonstrate that localization of Arp2/3 complex mRNAs in the leading protrusions is functionally important for directional cell migration.
- Subjects :
- Blotting, Western
macromolecular substances
Article
Cell Movement
medicine
Animals
RNA, Messenger
Cytoskeleton
Actin
Cells, Cultured
In Situ Hybridization
Cell Nucleus
Messenger RNA
Gene knockdown
Perinuclear endoplasmic reticulum
biology
Cell migration
Cell Biology
Fibroblasts
Cell biology
Cell nucleus
Protein Transport
medicine.anatomical_structure
Formins
Actin-Related Protein 3
Gene Knockdown Techniques
Actin-Related Protein 2
biology.protein
biological phenomena, cell phenomena, and immunity
Chickens
Subjects
Details
- ISSN :
- 10902422
- Volume :
- 317
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Experimental cell research
- Accession number :
- edsair.doi.dedup.....b2dc96221eae7a7c1cf7f3ccb42a918a