Back to Search
Start Over
The Streptomycin-Treated Mouse Intestine Selects Escherichia coli envZ Missense Mutants That Interact with Dense and Diverse Intestinal Microbiota
- Source :
- Infection and Immunity. 80:1716-1727
- Publication Year :
- 2012
- Publisher :
- American Society for Microbiology, 2012.
-
Abstract
- Previously, we reported that the streptomycin-treated mouse intestine selected nonmotile Escherichia coli MG1655 flhDC deletion mutants of E. coli MG1655 with improved colonizing ability that grow 15% faster in vitro in mouse cecal mucus and 15 to 30% faster on sugars present in mucus (M. P. Leatham et al., Infect. Immun. 73:8039–8049, 2005). Here, we report that the 10 to 20% remaining motile E. coli MG1655 are envZ missense mutants that are also better colonizers of the mouse intestine than E. coli MG1655. One of the flhDC mutants, E. coli MG1655 Δ flhD , and one of the envZ missense mutants, E. coli MG1655 mot-1, were studied further. E. coli MG1655 mot-1 is more resistant to bile salts and colicin V than E. coli MG1655 Δ flhD and grows ca. 15% slower in vitro in mouse cecal mucus and on several sugars present in mucus compared to E. coli MG1655 Δ flhD but grows 30% faster on galactose. Moreover, E. coli MG1655 mot-1 and E. coli MG1655 Δ flhD appear to colonize equally well in one intestinal niche, but E. coli MG1655 mot-1 appears to use galactose to colonize a second, smaller intestinal niche either not colonized or colonized poorly by E. coli MG1655 Δ flhD . Evidence is also presented that E. coli MG1655 is a minority member of mixed bacterial biofilms in the mucus layer of the streptomycin-treated mouse intestine. We offer a hypothesis, which we call the “Restaurant” hypothesis, that explains how nutrient acquisition in different biofilms comprised of different anaerobes can account for our results.
- Subjects :
- Immunology
Mutant
Mutation, Missense
Biology
medicine.disease_cause
Microbiology
Mice
chemistry.chemical_compound
Multienzyme Complexes
Escherichia coli
medicine
Animals
Selection, Genetic
Escherichia coli Proteins
Biofilm
Gene Expression Regulation, Bacterial
Bacterial Infections
Adaptation, Physiological
Mucus
In vitro
Intestines
Infectious Diseases
chemistry
Streptomycin
Biofilms
Galactose
Colicin
bacteria
Parasitology
Bacterial Outer Membrane Proteins
medicine.drug
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....b2e2f2bb21b9de0b928cfbd2cf285518
- Full Text :
- https://doi.org/10.1128/iai.06193-11