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Quality assessment of cryopreserved Portuguese oyster (Crassostrea angulata) sperm through ultrastructural and flow cytometry analysis
Quality assessment of cryopreserved Portuguese oyster (Crassostrea angulata) sperm through ultrastructural and flow cytometry analysis
- Source :
- Cryobiology. 104:79-86
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- We investigated the effects of cryopreservation on the quality of Portuguese oyster (Crassostrea angulata) sperm, which were examined before and after freezing; sperm motility, fertilizing capacity, and ultrastructural morphology were analyzed. The motility percentage and fertilizing capacity of the cryopreserved sperm (mean ± standard error) were 16% ± 1% and 17% ± 8%, respectively. In the pre-freezing sperm, these were 58% ± 2% and 76% ± 4%, respectively. The sperm sustained substantial morphological and ultrastructural damage during cryopreservation. The morphological changes varied considerably in nature and extent, ranging from no apparent damage to virtual disintegration. Sperm were stained with fluorescent dyes to assess viability, plasma membrane integrity, mitochondrial activity, acrosomal membrane integrity, oxidation level, and DNA fragmentation and examined through flow cytometry. The methods used for the flow cytometry assays were slightly modified from those used for evaluating the semen quality of livestock. Relative to the pre-freezing sperm, the frozen-thawed sperm exhibited lower acrosomal membrane integrity (acrosomal damage, 59.86 ± 5.29; P
- Subjects :
- Male
Oyster
General Biochemistry, Genetics and Molecular Biology
Cryopreservation
Andrology
Semen quality
biology.animal
Animals
Crassostrea
Sperm motility
Portugal
biology
urogenital system
General Medicine
Acrosomal membrane
Flow Cytometry
biology.organism_classification
Spermatozoa
Sperm
Semen Analysis
Sperm Motility
DNA fragmentation
General Agricultural and Biological Sciences
Semen Preservation
Portuguese oyster
Subjects
Details
- ISSN :
- 00112240
- Volume :
- 104
- Database :
- OpenAIRE
- Journal :
- Cryobiology
- Accession number :
- edsair.doi.dedup.....b2ec3212d8cae51e336a905a2e833561
- Full Text :
- https://doi.org/10.1016/j.cryobiol.2021.09.005