Back to Search
Start Over
Flow sorting and molecular cytogenetic identification of individual chromosomes of Dasypyrum villosum L. (H. villosa) by a single DNA probe
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 11, p e50151 (2012)
- Publication Year :
- 2012
-
Abstract
- Dasypyrum villosum (L.) Candargy (sin. Haynaldia villosa) is an annual wild diploid grass species (2n = 2x = 14; genome VV) belonging to the Poaceae family, which is considered to be an important source of biotic and abiotic stress resistance genes for wheat breeding. Enhanced characterization of D. villosum chromosomes can facilitate exploitation of its gene pool and its use in wheat breeding programs. Here we present the cytogenetic identification of D. villosum chromosomes on slide by fluorescent in situ hybridization (FISH), with the GAA simple sequence repeat (SSR) as a probe. We also describe the isolation and the flow cytometric analysis of D. villosum chromosomes in suspension, resulting in a distinguished flow karyotype. Chromosomes were flow sorted into three fractions, according their DNA content, one of which was composed of a single type of chromosome, namely 6 V, sorted with over 85% purity. Chromosome 6 V is known to carry genes to code for important resistance and seed storage characteristics, and its isolation represents a new source of genetic traits and specific markers useful for wheat improvement.
- Subjects :
- Cytometric Techniques
RIBOSOMAL-RNA GENES
lcsh:Medicine
Plant Science
Dasypyrum villosum
Plant Genetics
Genome
TRITICUM-AESTIVUM L
Molecular Cell Biology
Plant Genomics
Genome Sequencing
lcsh:Science
In Situ Hybridization, Fluorescence
Triticum
STREAK-MOSAIC-VIRUS
Genetics
PLANT CHROMOSOMES
Multidisciplinary
biology
Chromosome Biology
Hybridization probe
food and beverages
HAYNALDIA-VILLOSA
Karyotype
Genomics
SIMPLE SEQUENCE REPEATS
Flow Cytometry
Cytogenetic Analysis
Gene pool
Ploidy
Cytological Landmarks
DNA Probes
Genome, Plant
Research Article
DNA, Plant
Poaceae
Chromosomes, Plant
Cytogenetics
Gene
Biology
Ploidies
lcsh:R
TRANSLOCATION LINES
Chromosome
BREAD WHEAT
IN-SITU HYBRIDIZATION
CONFERRING RESISTANCE
biology.organism_classification
Agronomy
Plant Breeding
Karyotyping
Hybridization, Genetic
lcsh:Q
Plant Biotechnology
Cytogenetic Techniques
Cytometry
Microsatellite Repeats
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....b2fde7b0535e905094b5b90ff37c52b0