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DNA content and chromatin texture of human breast epithelial cells transformed with 17-β-estradiol and the estrogen antagonist ICI 182,780 as assessed by image analysis
- Source :
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 617:1-7
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- The immortalized human breast epithelial MCF-10F cell line, although estrogen receptor alpha negative, develops cell proliferating activities and invasiveness indicative of neoplastic transformation, after treatment with 17-beta-estradiol (E-2). These effects are similar to those produced by benzo[a]pyrene (BP). Since we have previously reported changes in the nuclear parameters accompanying BP-induced tumorigenesis in MCF-10F cells, we have examined whether similar alterations occur in E-2-treated cells. We therefore studied DNA amounts and other nuclear parameters in Feulgen-stained MCF-10F cells after treatment with various concentrations of E-2, BP, the estrogen antagonist ICI 182,780, and E-2 in the presence of ICI 182,780. E-2 caused a certain loss of DNA and changes in the nuclear size and chromatin supraorganization of MCF-10F cells. Many of these changes were similar to those produced by BP and were indicative of neoplastic transformation. More intense chromatin remodelling was seen with 70 nM E-2. Since these changes were not abrogated totally or partially by ICI 182,780, the neoplastic transformation of MCF-10F cells stimulated by E-2 involved a process that was independent of estrogen alpha-receptors. The changes produced by ICI 182,780 alone were attributed to effects other than its well-known anti-estrogenic activity.
- Subjects :
- medicine.medical_specialty
medicine.drug_class
Health, Toxicology and Mutagenesis
Cell
Breast Neoplasms
Biology
medicine.disease_cause
Article
Internal medicine
Benzo(a)pyrene
Genetics
medicine
Humans
Neoplastic transformation
Breast
skin and connective tissue diseases
Fulvestrant
Molecular Biology
Cell Line, Transformed
Image Cytometry
Cell Nucleus
Estradiol
Estrogen Antagonists
Epithelial Cells
DNA, Neoplasm
Molecular biology
Chromatin
Cell Transformation, Neoplastic
medicine.anatomical_structure
Endocrinology
Estrogen
Cell culture
Female
Carcinogenesis
Estrogen receptor alpha
medicine.drug
Subjects
Details
- ISSN :
- 00275107
- Volume :
- 617
- Database :
- OpenAIRE
- Journal :
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
- Accession number :
- edsair.doi.dedup.....b301e9085ae7d77edbbce3687f85b989